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Process for preparing chitosan oligosacharides

A technology for chitosan oligosaccharide and chitosan, which is applied in the field of chitosan oligosaccharide production to achieve the effects of high yield, good water solubility and low cost

Inactive Publication Date: 2006-12-27
青岛弘海生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of this invention is to provide a kind of method for producing oligochitosan, to overcome the above-mentioned shortcoming of existing enzymatic production oligochitosan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] (1) Acquisition of chitosanase-producing strains: first prepare selective plate culture medium: weigh chitosan 1.0g, (NH 4 ) 2 SO 4 0.5g, yeast extract powder 0.1g, K 2 HPO 4 0.05g, KH 2 PO 4 0.3g, NaCl 1.0g, MgSO 4 ·7H 2 O 0.05g, agar 2.0g, placed in a 500ml Erlenmeyer flask, measure 95g of distilled water, pour it into the above Erlenmeyer flask, adjust the pH to 8.1 with 1mol / L NaOH aqueous solution, add a cotton plug, and extinguish at 121°C under high pressure. Bacteria 20min. After sterilization, divide the prepared culture medium into glass plates with a diameter of 9 cm, 15 ml in each plate, and cool to room temperature to obtain a selective plate culture medium. Take 0.2ml of seawater in the shrimp pond, spread it evenly on the above-mentioned selective plate medium under aseptic conditions, and cultivate it for 5 days at 28°C to obtain chitosanase-producing bacteria. The above-mentioned chitosanase-producing bacteria were further purified by a strea...

Embodiment 2

[0017] (1) Obtaining of the chitosanase-producing strain: the same as the step of (1) in Example 1.

[0018] (2) Preparation of chitosanase fermentation broth: the same as the step of (2) in Example 1.

[0019] (3) Preparation of chitosan enzyme powder: Centrifuge the chitosan enzyme fermentation broth at 5000rpm for 30min at 4°C to remove bacteria, obtain the centrifuged supernatant enzyme liquid, and then ultrafilter it through an ultrafiltration membrane with a molecular weight cut-off of 10000Da Remove the small molecular substances to obtain the concentrated enzyme solution containing large molecular weight, which is freeze-dried to obtain chitosan enzyme powder.

[0020] (4) Preparation of chitosan hydrolyzate: weigh 15Kg of chitosan and place it in a 500L enamel tank, add 294L of water, 10L of acetic acid, stir to dissolve, add 25g of chitosan enzyme powder, stir and degrade at 40°C for 15 hours , to obtain chitosan enzymatic hydrolysis solution.

[0021] In the prese...

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PUM

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Abstract

The invention discloses a method for preparing Chitosan oligosaccharide, which is characterized in that: dissolving Chitosan oligosaccharide into acetate solution, adding Chitosan oligosaccharide enzyme for hydrolysis; condensing got hydrolysate, depositing with ethanol, drying or spraying for drying; said Chitosan oligosaccharide enzyme is Chitosan oligosaccharide enzyme liquid or powder; the ratio between volume of said Chitosan oligosaccharide enzyme liquid and weight of Chitosan oligosaccharide is 0.1-2 l :1 kg; and the weight ratio between powder and Chitosan oligosaccharide is 0.5 g- 10 g: 1 kg. The Chitosan oligosaccharide prepared in this invention is characterized by good water solubility, high productivity and low cost.

Description

technical field [0001] The invention relates to a method for producing chitosan oligosaccharide, in particular to a method for producing chitosanase by fermenting and cultivating chitosanase-producing microorganisms, and using the enzyme to degrade chitosan to produce chitosan oligosaccharide. Background technique [0002] Chitin and chitosan are one of the research hotspots in the field of polysaccharides. Chitosan is the product of chitin deacetylation and has broad application prospects in industry, agriculture, food, medicine and other fields. However, because chitosan is insoluble in water, it is not easy to degrade and absorb in the body, thus affecting its application range. Oligochitosan is a glucosamine oligosaccharide with a polymerization degree of 2 to 20 formed after the degradation of chitosan. It has good water solubility and has many unique physiological activities and functions, such as enhancing the body's immunity and inhibiting tumor metastasis. , lower ...

Claims

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Application Information

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IPC IPC(8): C08B37/08C07H3/06C12P19/04
Inventor 刘万顺韩宝芹彭睿
Owner 青岛弘海生物技术有限公司
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