Hemoglobin chip preparation and electrochemical detecting method thereof

Abstract

The preparation method for hemoglobin chip comprises: 1) preparing the print carbon electrode; 2) preparing and modifying the Prussian blue nano particle; 3) evaporating and drying the particle to dissolve into 0.1M phosphate buffer solution with pH value as 7.0 and spraying on the electrode surface, drying in icebox for standby. Besides, the electrochemical detection method comprises: 1) optimizing the test condition: a) preparing standard solution; b) selecting detection potential; c) setting pH value for the buffer solution; 2) plotting the normal curve; 3) correcting the curve. This invention is simple and fast with low cost.

Description

technical field [0001] The invention relates to the preparation of a hemoglobin clinical detection chip, in particular, a method for measuring hemoglobin content in blood is established in combination with electrochemical analysis technology. Background technique [0002] Hemoglobin (Hemoglobin) is the respiratory protein in the red blood cells of vertebrates. It is the main substance that transports oxygen in the blood. It plays the role of transporting oxygen and decomposing H 2 o 2 Important activities related to oxygen and energy metabolism, such as transfer of electrons, play a key role in all life activities. The relative molecular mass of hemoglobin is about 67,000, and its molecule has a quaternary structure. It is a tetramer composed of two α and two β polypeptide chains. Each peptide chain is bound to a heme molecule and is close to each other. Form approximately spherical hemoglobin molecules with a diameter of about 55nm. Heme is located in a hydrophobic envir...

AI Technical Summary

Problems solved by technology

All these methods require special reagents and spectroscopic instruments, and the operation is complicated and costly, which is not conducive to popularization and use.