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Method, reagent, and kit for determining cholesterol in very-low-density lipoprotein remnant (vldl remnant)

An extremely low-density lipoprotein technology, applied in biochemical equipment and methods, microbiological determination/inspection, biological testing, etc., can solve the problems of complicated operation of ultracentrifugation

Inactive Publication Date: 2007-02-14
KYOWA MEDEX CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the operation of this ultracentrifugation method is very complicated

Method used

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  • Method, reagent, and kit for determining cholesterol in very-low-density lipoprotein remnant (vldl remnant)
  • Method, reagent, and kit for determining cholesterol in very-low-density lipoprotein remnant (vldl remnant)
  • Method, reagent, and kit for determining cholesterol in very-low-density lipoprotein remnant (vldl remnant)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0277] Example 1 VLDL Residue-C Quantification Kit

[0278] A kit for quantifying VLDL remnants-C containing the following first and second reagents was prepared.

[0279] ·The first reagent

[0280] MOPS(pH6.5) 20mmol / L

[0281] TOOS 0.3g / L

[0282] Sodium sulfate 1g / L

[0283] Emulsifier L-40 5g / L

[0284] Prolon B-208 6g / L

[0285] Peroxidase (POD) 10U / mL

[0286] Ascorbate oxidase (AOD) 2U / mL

[0287] ·Second reagent

[0288] MOPS(pH6.8) 20mmol / L

[0289] 4-Aminoantipyrine 0.5g / L

[0290] Emulsifier L-40 2g / L

[0291] Peroxidase (POD) 10U / mL

[0292] Lipoprotein lipase (LPL) 2U / mL

[0293] Cholesterol oxidase 2U / mL

Embodiment 2

[0294] Embodiment 2 VLDL remnants-C quantification kit

[0295] A kit for quantifying VLDL remnants-C containing the following first and second reagents was prepared.

[0296] ·The first reagent

[0297] MOPS(pH6.5) 20mmol / L

[0298] TOOS 0.3g / L

[0299] Sodium sulfate 1g / L

[0300] BLAUNON TSP-50 7g / L

[0301] BSA 1g / L

[0302] Peroxidase (POD) 10U / mL

[0303] Ascorbate oxidase (AOD) 2U / mL

[0304] ·Second reagent

[0305] MOPS(pH6.8) 20mmol / L

[0306] 4-Aminoantipyrine 0.5g / L

[0307] Emulsifier L-40 2g / L

[0308] Peroxidase (POD) 10U / mL

[0309] Lipoprotein lipase (LPL) 2U / mL

[0310] Cholesterol oxidase 2U / mL

Embodiment 3

[0311] Embodiment 3 VLDL remnants-C quantification kit

[0312] A kit for quantifying VLDL remnants-C containing the following first and second reagents was prepared.

[0313] ·The first reagent

[0314] MOPS(pH6.8) 20mmol / L

[0315] TOOS 0.3g / L

[0316] Sodium sulfate 1g / L

[0317] ユニル一ブMT-0620B 6g / L

[0318] Peroxidase (POD) 10U / mL

[0319] Ascorbate oxidase (AOD) 2U / mL

[0320] ·Second reagent

[0321] MOPS(pH6.8) 20mmol / L

[0322] 4-Aminoantipyrine 0.5g / L

[0323] Emulsifier L-40 2g / L

[0324] Peroxidase (POD) 10U / mL

[0325] Lipoprotein lipase (LPL) 2U / mL

[0326] Cholesterol oxidase 2U / mL

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PUM

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Abstract

Ester-form and free-form cholesterols contained in a very-low-density lipoprotein remnant (hereinafter, inclusively referred to as very-low-density lipoprotein remnant cholesterols) are determined. In an aqueous medium containing a sample, a cholesterol ester hydrolase and a cholesterol oxidase or cholesterol dehydrogenase are caused to act specifically on the very-low-density lipoprotein remnant cholesterols in the sample in the presence of a combination of (a) a polyoxyethylene-polyoxyalkylene alkylaryl ether and (b) at least one surfactant selected from the group consisting of a polyoxyethylene / polyoxybutylene condensate, a polyoxyethylene styrenated phenyl ether, and a polyoxyalkylene long-chain alkyl ether.

Description

technical field [0001] The invention relates to a method, reagent and kit for quantifying cholesterol in very low-density lipoprotein remnants (VLDL remnants) in a sample. Background technique [0002] Blood contains various lipoproteins. According to different specific gravity, these lipoproteins are classified into chylomicrons (hereinafter referred to as CM), very low density lipoprotein (hereinafter referred to as VLDL), low density lipoprotein (hereinafter referred to as LDL) and high density lipoprotein (hereinafter referred to as HDL). ). Various lipoproteins have different proportions of components such as cholesterol, triglycerides, phospholipids, and proteins, and have different functions in the living body. [0003] In addition to these lipoproteins, blood also contains remnant lipoproteins (remnant lipoproteins) produced during lipoprotein metabolism and decomposition by lipase. Residual lipoproteins are classified into chylomicron remnants (hereinafter referr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/60C12Q1/26C12Q1/28C12Q1/32C12Q1/44G01N33/92C12Q1/34
CPCC12Q1/60C12Q1/34G01N33/92G01N2800/044
Inventor 宫内一人
Owner KYOWA MEDEX CO LTD
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