Traditional Chinese medicinal formulation for treating cardiovascular and cerebrovascular disease
A technology of cardiovascular and cerebrovascular diseases and traditional Chinese medicine, which is applied in the field of traditional Chinese medicine compositions for the treatment of cardiovascular and cerebrovascular diseases. It can solve the problems of being unable to take care of oneself, the burden of relatives and society, and achieve the effects of quality control, easy industrialization, and multiple benefits.
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Embodiment 1
[0100] Preparation of Danshensu extract: take Danshen decoction pieces, add water 5 times the weight of the crude drug, add 0.4% aqueous sodium bicarbonate solution to adjust the pH to 8, reflux extraction for 2 hours, and separate the extract; add 5 times the weight of the crude drug to the residue amount of water, reflux extraction for 2 hours, separate the extract, combine the two extracts, and filter; add 1.5% hydrochloric acid to the filtrate to adjust the pH of the solution to 2, filter or centrifuge, and concentrate the supernatant or filtrate under reduced pressure to a density of 1.08 (55-60°C); add 95% ethanol to the concentrated solution to make the alcohol concentration of the solution 50%, let it stand for 18 hours, filter, and concentrate the filtrate under reduced pressure until there is no alcohol smell; AB-8 type large Pore resin (produced by Tianjin Nankai University Resin Factory) column, first wash away with water of 2 times the column volume, discard, the...
Embodiment 2
[0104] Preparation of Danshensu extract: take Danshen decoction pieces, crush them, add water 5 times the weight of the crude drug, add 0.35% sodium carbonate to adjust the pH to 9, extract by ultrasonic for 30 minutes, separate the extract; the residue is 5 times the weight of the crude drug amount of water, ultrasonic extraction for 20 minutes, separate the extract, combine the two extracts, and filter; add 2% acetic acid to the filtrate to adjust the pH of the solution to 2, filter or centrifuge, and concentrate the supernatant or filtrate under reduced pressure to a density of 1.06 (55-60°C); add 95% ethanol to the concentrated solution to make the alcohol concentration of the solution 40%, let it stand for 18 hours, filter, and concentrate the filtrate under reduced pressure until it has no alcohol smell; put D101 macroporous resin on the concentrated solution (produced by Resin Factory of Nankai University, Tianjin) column, wash off with water of 2 times column volume fir...
Embodiment 3
[0108] Preparation of Danshensu extract: take Danshen decoction pieces, crush them, add water 5 times the weight of the crude drug, add 0.35% sodium carbonate to adjust the pH value to 9, reflux extraction for 2 hours, separate the extract; the residue is 5 times the weight of the crude drug amount of water, reflux extraction for 1.5 hours, separate the extract, combine the two extracts, and filter; add 1% hydrochloric acid to the filtrate to adjust the pH of the solution to 2, filter or centrifuge, and concentrate the supernatant or filtrate under reduced pressure to a density of 1.06 (55-60°C); add 95% ethanol to the concentrated solution to make the alcohol concentration of the solution 65%, let it stand for 18 hours, filter, and concentrate the filtrate under reduced pressure to no alcohol smell; polyamide (Qingdao Haiyang Chemical Plant) column, collect the eluate; the eluate is then loaded on the ZTC type macroporous resin (produced by Tianjin Nankai University Resin Fact...
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