The invention provides a fulvic acid-containing large-medium-trace element water-soluble fertilizer and a preparation method thereof, wherein the fulvic acid-containing large-medium-trace element water-soluble fertilizer comprises, by mass, 50-90% of fulvic acid, and 10-50% of water-soluble elements, wherein the water-soluble elements comprise large water-soluble elements of potassium dihydrogen phosphate and urea, medium water-soluble water-soluble elements of ammonium calcium nitrate and magnesium sulfate, trace water-soluble elements of zinc sulfate, manganese sulfate, ferrous sulfate, copper sulfate and ammonium tetramolybdate, alginic acid and a chelating agent. Compared with the preparation method in the prior art, the preparation method of the present invention has the following characteristics that fulvic acid is produced by using local straw, rape, corn stalk herb waste as raw materials, and a variety of large-medium-trace elements capable of improving the absorption rate of crops are added, such that the fulvic acid-containing large-medium-trace element water-soluble fertilizer is prepared, the environment is improved, the cost of the fulvic acid water-soluble fertilizeris reduced, the benefits of enterprises are increased, and the competitiveness of enterprises is enhanced.