The invention discloses an extracting process of pulullan
polysaccharide and belongs to the technical field of
biology. The extracting process mainly comprises the following steps of: (1), filtering by a plate frame to remove
bacteria; (2), performing
thermal treatment to remove
protein; (3), carrying out primary ultra-
filtration; (4), removing
ion by use of
ion exchange resin; (5), discoloring
active carbon; (6), carrying out secondary ultra-
filtration; and (7),
drying, crushing and packaging. According to the extracting process of the pulullan
polysaccharide disclosed by the invention, the
bacteria in
fermentation liquor are removed by adopting a plate frame filter, so that not only can
solvent residue caused by adding an organic flocculating agent and inorganic flocculating agent be effectively avoided, but also the
bacteria and diatomite can be recycled to obtain more benefits; meanwhile, potential unsafe factors caused by volatility of solvents (
ethanol, methyl
alcohol, isopropanol and the like) in the conventional process can be avoided by adopting a
microwave drying process or a
vacuum drying process; moreover, much steam consumed by recycling and distilling an
organic solvent can be saved; the production cost is saved; the product yield can be up to 90%-96%, the purity is as high as 90%-98%, the quality is good, and the production process is safe, continuous and automatic.