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Extraction method for primary active matter of edible fungi

An extraction method and technology for active substances, which are applied in the field of preparation of edible fungus extracts, can solve the problems of loss of active ingredients, complexation, hydrolysis, oxidation, reduction, combustion, etc., and achieve the effects of reducing volatilization and avoiding interference and harm.

Inactive Publication Date: 2007-04-18
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many obvious defects in the traditional extraction method: 1. Active substances are water-soluble (such as: alkaloid salts, flavonoids, etc.), alcohol-soluble (such as: oil, sterol, etc.), and fat-soluble (such as: terpene class, free alkaloids, glycosides, etc.), so a single extraction solution only extracts part of the active substances, resulting in the loss of other active ingredients; 2. Complexation, hydrolysis, oxidation, reduction, etc. Chemical reaction changes the original components; 3. When water is used as a solvent for extraction at room temperature, the extract is prone to enzymatic hydrolysis and deterioration due to microbial reproduction; 4. Petroleum ether and ethanol are volatile when the temperature is high, and even steam occurs burning hazard

Method used

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  • Extraction method for primary active matter of edible fungi
  • Extraction method for primary active matter of edible fungi
  • Extraction method for primary active matter of edible fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Extraction of the active components of Ganoderma lucidum

[0032] In an air-conditioned workshop at 18°C, add 1 kg of whole Ganoderma lucidum (Ganoderma lucidum processed product, powder) (produced by Guangdong Yuewei Edible Fungi Technology Co., Ltd.) into 3L of petroleum ether, soak in an ultrasonic washing tank for 1 hour, filter, and add 1L of petroleum ether to the filter residue Filter after soaking, repeat the operation once, combine the filtrate and concentrate with ultrafiltration equipment to obtain a concentrated solution, and the product (A) after the concentrated solution is dried by a freeze dryer is temporarily stored in a drying dish. After the filter residue was dried in the shade for 1 hour, add 3L of 95% ethanol and soak it in an ultrasonic washing tank for 2 hours, filter, add 1L of ethanol to the filter residue twice for soaking and then filter, combine the filtrate and concentrate it with ultrafiltration equipment to obtain a concentrated solutio...

Embodiment 2

[0038] 1. Extraction of active components of Versicolor versicolor

[0039] The dried fruiting body (mushroom body) of Yunzhi was pulverized at 18°C ​​with a water-cooled low-temperature pulverizer equipped with a 40-mesh sieve. In an air-conditioned workshop at 18°C, add 1 kg of Versicolor versicolor fruiting body powder to 1.5 L of petroleum ether and immerse in an ultrasonic washing tank for 1 hour, filter, and filter the filter residue after soaking with 1 L of petroleum ether, repeat the operation once, and combine the filtrates with ultrafiltration The equipment is concentrated to obtain a concentrated solution, and the product (A) after the concentrated solution is dried by a freeze dryer is temporarily stored in a drying dish. After the filter residue was dried in the shade for 1 hour, add 1.5L 95% ethanol and immerse in the ultrasonic washing tank for 2 hours, filter, add 1L ethanol to the filter residue twice and then filter, combine the filtrate and concentrate it w...

Embodiment 3

[0045] 1. Extraction of Active Components of Lentinus edodes

[0046]Fresh shiitake mushrooms were dehydrated in quick-freezing equipment for 6 hours, and the fruiting bodies (mushroom bodies) of shiitake mushrooms were pulverized at 18°C ​​with a water-cooled low-temperature pulverizer equipped with a 20-mesh sieve. In an air-conditioned workshop at 18°C, add 1kg of shiitake fruiting powder to 2L of petroleum ether, soak in an ultrasonic washing tank for 1 hour, filter, add 1L of petroleum ether to the filter residue twice and then filter, combine the filtrate and concentrate it with ultrafiltration equipment A concentrated solution is obtained, and the product (A) after the concentrated solution is dried by a freeze dryer is temporarily stored in a drying dish. After the filter residue was dried in the shade for 1 hour, add 2L of 95% ethanol and immerse in an ultrasonic washing tank for 2 hours, filter, add 1L of ethanol to the filter residue twice for immersion and then fil...

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Abstract

A low-temp (lower than 20 deg.C) process for extracting the original-state active components from edible fungus includes such steps as dewatering, breaking, extracting in oil, extracting in alcohol, extracting in water, ultrafiltering, drying, mixing and cold storage.

Description

【Technical field】 [0001] The invention belongs to the field of preparation of edible fungus extracts. 【Background technique】 [0002] Edible fungi contain a variety of biologically active components, including polysaccharides, triterpenes, sterols, glycosides, macrolides, polypeptides, alkaloids, flavonoids, oils and other components. Because these active substances have many functions such as anti-oxidative aging, improving immunity, and preventing cardiovascular diseases, they are increasingly favored by the public. Therefore, the research on the extraction methods of active substances in edible fungi has also attracted widespread attention. The traditional extraction methods mainly include decoction method and dipping method. The decoction method is to crush the material and add appropriate amount of water according to the texture, fully infiltrate for 1 to 2 hours, then decoct and boil for 1 to 2 hours, and the residue after coarse filtration Repeat the above steps 2 to...

Claims

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Application Information

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IPC IPC(8): A61K36/06A61P9/00A61P39/06A23L31/00
Inventor 杨小兵
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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