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Culture medium formulation for fermentation production of lactic acid

A culture medium and technology of lactic acid bacteria, applied in the biological field, can solve problems such as uncertain growth factor composition and content, low lactic acid production and yield, and many by-products

Active Publication Date: 2007-05-16
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the nutritional requirements of bacterial homozyme fermentation are relatively high. Although the yeast extract contained in the culture medium usually contains trace amounts of essential amino acids and growth factors, the composition and content of the growth factors are uncertain and cannot fully meet the needs of lactic acid fermentation.
[0004] In the prior art, the original medium formula is peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, K 2 HPO 4 2g / L, diamine citrate 2g / L, sodium acetate 4g / L, glucose 40g / L, MgSO 4 ·7H 2 O0.2g / L, MnSO 4 ·H 2 O 0.2g / L, ferrous sulfate 0.01g / L, sodium chloride 0.03g / L, Tween-801 mL / L, adjust pH6.0, the by-product of this formula is more, and the output of lactic acid and productive rate are relatively The main reason is that the growth and acid production of bacteria are affected by the lack of growth factors

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment approach

[0150] As a preferred embodiment of the present invention, the components and contents of the medium are:

[0151] Thiamine 0.005-0.1mg / L, riboflavin 0.005-0.2mg / L, niacin 0.1-20mg / L, pyridoxal hydrochloride 0.01-4.0mg / L, biotin 0.001-1.0mg / L, peptone 1 -50g / L, beef extract 1-50g / L, yeast extract 0.5-50g / L, K 2 HPO 4 0.2-20g / L, diamine citrate 0.2-20g / L, sodium acetate 0.2-20g / L, glucose 10-500g / L, MgSO 4 ·7H 2 O 0.005-2g / L, MnSO 4 ·H 2 O0.005-2g / L, ferrous sulfate 0.0001-1g / L, sodium chloride 0.0005-1g / L, Tween-800.005-10mL; use NaOH to adjust the pH to 5.0-7.0.

[0152] The medium of the present invention is suitable for any lactic acid bacteria used in the field to ferment and produce lactic acid, including but not limited to: Lactobacillus lactis (L.Lactis) and Lactobacillus casei (L.casei); for example, the medium can be used for culturing : Lactobacillus paracasei (Lactobacillus paracasei), can use Lactobacillus paracasei (L.casei) to biosynthesize lactic acid.

...

Embodiment 1

[0159] The formula of embodiment 1 medium

[0160] Several formulations of the medium are shown in Table 1:

[0161] Table 1

[0162] Medium 1

[0163] Ferrous sulfate (g / L)

Embodiment 2

[0164] Embodiment 2 adopts culture medium 1 to produce lactic acid

[0165] 1. Preparation of culture medium 1

[0166] 1) According to peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, K 2 HPO 4 2g / L, diamine citrate 2g / L, sodium acetate 4g / L, MgSO 4 ·7H 2 O 0.2g / L, MnSO 4 ·H 2 O 0.2g / L, Ferrous Sulfate 0.01g / L, Sodium Chloride 0.03g / L, Tween-80 1mL / L Weigh the above medium components and add appropriate amount of distilled water to dissolve; use 4mol / L NaOH solution Adjust the pH to 6.0, and sterilize at 121°C for 20 minutes.

[0167] 2) According to the final concentration of glucose in the culture medium being 200g / L, prepare a certain concentration of mother liquor in proportion, and sterilize at 115°C for 20min.

[0168] 3) According to the final concentration of combined vitamins in the medium: thiamine 0.053mg / L, riboflavin 0.01mg / L, niacin 4.0mg / L, pyridoxal hydrochloride 0.2mg / L, biotin 0.075mg / L L, prepare a mother liquor with a certain concentration, ...

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Abstract

The invention discloses a culture medium and utility and manufacturing method to produce lactic acid, which comprises the following parts: fitful thiamine, lactochrome, niacin, alcaine pyridoxal and biotin.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a lactic acid bacteria culture medium and a lactic acid production method using the lactic acid bacteria culture medium. Background technique [0002] Lactic acid is an important industrial organic acid, which has a wide range of applications, and can be widely used in food, pharmaceuticals, leather, textiles, environmental protection and agriculture. The main forms of its products are sour agents, flavoring agents, preservatives, tanning agents, plant growth regulators, biodegradable materials and chiral drugs, etc. Because lactic acid is widely used and has a tendency to expand continuously, the development of lactic acid derivatives is increasing year by year, so the demand for lactic acid in the world is increasing rapidly. [0003] Different strains have different lactic acid fermentation mechanisms, which can be divided into two types: homozygous fermentation and h...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/225
Inventor 庄英萍邱伙琴徐国谦储炬张嗣良王永红
Owner EAST CHINA UNIV OF SCI & TECH
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