Rice breeding method incorporating high photosynthetic efficiency and presenility resistant property

A high-light-efficiency, rice-based technology, applied in the fields of botanical equipment and methods, introduction of foreign genetic material using vectors, plant genetic improvement, etc., can solve the problems of low photosynthetic efficiency, easy aging, etc. Effects of Genetic Disorders

Inactive Publication Date: 2007-07-11
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is: aiming at the low photosynthetic efficiency and easy senescence commonly existing in rice at present, especially the practical problems that senescence directly

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1 Transfer of γ-ECS gene to Suhu Xiangjing

[0023] In 2003, the γ-ECS gene of maize was transferred. The specific method is as follows: The mature seeds of Suhuxiangjing rice were dehulled, treated with 75% alcohol for 1 minute, and then soaked in 0.1% mercuric chloride solution for 15 minutes. Rinse the bacteria water 3 times and inoculate it on the callus induction medium with M8 as the basic medium, hormone type and ratio (the formula for inducing mature embryo callus is M8+2mg / L2, 4-D1mg / LABA +1 mg / L6-BA, 3% sucrose + 7% agar) cultured in the dark at 28°C for 30 days. The yellow-white compact callus was cut out from the seed embryo scutellum, and then immersed in the bacterial solution of Agrobacterium strain GV3010 carrying the γ-ECS gene for 5 minutes, then co-cultured for 48 hours, and then connected with 500mg / In the selection medium of L cephalosporin and 50 mg / L kanamycin (except for antibiotics, the selection medium has the same ingredients as the matur...

Example Embodiment

[0030] Example 2 Obtaining F1 from γ-ECS-Suhuxiangjing / PEPC transgenic rice

[0031] In 2004, the anti-premature aging material H obtained in Example 1 2 The germplasm is used as the female parent, and it is obtained according to the method disclosed in the ZL01138012.8 patent application by Jiao Demao, Gao Dongying, Li Xia, etc., with C 4 The rice germplasm with stable expression of the plant PEPC gene is the male parent, and 112 hybrid F1 seeds were obtained through cross-breeding.

Example Embodiment

[0032] Example 3 F1 screening with high photosynthetic efficiency and anti-premature aging properties

[0033] All 112 hybrid F1 materials obtained in Example 2 were planted in Hainan in the winter of 2004. The PEPC activity and photosynthetic rate of the leaves were identified at the heading stage, and the chlorophyll content and chlorophyll content of the flag leaf at the 7th and 21st days after flowering were measured at the flowering stage. Fv / Fm, 55 rice materials F with high photosynthetic efficiency and anti-premature aging were screened out 1 Materials, the PEPC activities of the selected materials are all between 260~1099μmol / mgChl.h, and the photosynthetic rate value is between 17.2~29.8μmol / m 2 Between .s, Fv / Fm attenuation is between 5% and 10%, and chlorophyll content is attenuation between 12 and 20%. Both have high photosynthetic efficiency and anti-premature aging characteristics, which proves that a combination of two excellent physiological characteristics can be...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a rice seeding method which comprises that: leading decay gene into rice seed to obtain the rice system; crossing the rice with C4 plant PEPC gene stable representation with the rice system, to obtain F1 system; checking, selecting the rice with high light effect and anti-decay character; cultivating F1, to obtain two flower systems; checking, selecting the rice pure system with high light effect and anti-decay character.

Description

technical field [0001] The invention relates to the field of molecular physiological breeding, in particular to a rice breeding method that integrates high light efficiency and anti-aging traits by organically combining genetic engineering, conventional breeding and physiological identification. Background technique [0002] The dwarfing of rice varieties and the three-line matching of indica hybrid rice are two important milestones in the history of modern rice breeding, and they have greatly increased rice yield. The average yield of rice in my country has reached 6t / hm 2 , Jiangsu has exceeded 8t / hm 2 , the leaf area index is about 8-10, and it is quite difficult to increase the yield by increasing the leaf area of ​​the population. The light energy utilization rate of the existing high-yield rice varieties is analyzed at 1-1.5%, while the ideal light energy use rate can reach 3-5%. It can be seen that improving the photosynthetic efficiency of rice may be one of the ef...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H1/02A01H3/00A01H5/00C12N15/82
Inventor 李霞周月兰王超孙志伟郭士伟何冰焦德茂
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap