Methods and compositions for the detection of cervical cancer
a technology of cervical cancer and compositions, applied in drug compositions, instruments, peptides, etc., can solve the problems of less serious clinical problems, poorly defined molecular characterization of specific nuclear matrix proteins, etc., and achieve the effects of reducing in vivo expression of target protein, and reducing in vivo expression of target cervical cancer-associated protein
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Image
Examples
example 1
Isolation Of Cervical Cancer-Associated Nuclear Matrix Proteins from Cervical Cancer Tissue Samples and Cell Lines
[0115] Cervical cancer-associated proteins were identified by comparing silver stained 2-D gel patterns of proteins isolated from normal and cancerous cervical cells.
[0116] Fresh cervical carcinoma tissue was obtained from patients undergoing hysterectomy for clinically localized (stage IB, II or III, International Federation of Gynecology and Obstetrics or FIGO classification) carcinomas of the cervix from the Instituto Nacional de Cancerologia in Mexico City, Mexico, in accordance with Scientific and Ethics Committee Review Board approval. A small number of tumor tissues were obtained under Institutional Review Board approval from the Pittsburgh Cancer Center (Pittsburgh, Pa.). Normal cervical tissue was obtained under Institutional Review Board approval from patients undergoing hysterectomy for causes unrelated to abnormal cervical histopathology, via the Cooperativ...
example 2
Identification of Cervical Cancer-associated Nuclear Matrix Proteins Having Differential Appearance on 2-D Gels
[0125] As described in the previous Example, 2-D gel electrophoresis patterns were obtained by fractionating proteins isolated from either normal or cancerous cervical cells. FIG. 1a shows a typical cervical cancer-associated nuclear matrix protein pattern obtained from cervical cancer tissue. FIG. 1b shows a typical gel pattern produced by nuclear matrix proteins obtained from a normal cervical tissue sample. Approximately 600 proteins were resolved per gel. Most of the proteins observed were always present, irrespective of the type of cervical tissue under investigation.
[0126] Comparison of FIGS. 1 and 2 reveals that, while most proteins in the cancer and non-cancer samples are identical, there are five proteins that are unique to the cervical cancer sample (labeled in FIG. 1). The proteins, designated CvC-1 through CvC-5, were detected in 20 tissue samples obtained fro...
example 3
Characterization of Cervical Cancer-Associated Nuclear Matrix Protein Markers
[0131] Two protein staining spots detectable on a 2-D gel corresponding to CvC-3 and CvC-5 were isolated, the protein harvested and subjected to microsequence analysis, as described hereinbelow.
[0132] For sequencing of the cervical cancer-associated polypeptides CvC-3 and CvC-5, the nuclear matrix fraction from HeLa cells were electrophoresed on two-dimensional gels as described above. Each gel was loaded with 300 micrograms of protein isolated by the nuclear matrix protein isolation procedure, as described above. Following the second-dimension of electrophoresis, proteins were visualized by reverse staining. Briefly, gels were soaked in 200 mM imidazole for 0.10 minutes, rinsed for 1 minute in water, followed by 1-2 minutes in 300 mM zinc chloride (Fernandez-Patron et al. (1992) BioTechniques 12: 564-573). After the protein-containing spots began to appear, the gels were placed in water, and the relevant...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Mass | aaaaa | aaaaa |
| Mass | aaaaa | aaaaa |
| Mass | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More