Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Isoflavone-beta-D-glucan produced by Agaricus blazei in the submerged liquid culture and method of producing same

a technology of isoflavone-beta-d-glucan and liquid culture, which is applied in the field of isoflavone-beta-d-glucan produced by submerged liquid culture of agaricus blazei, can solve the problems of lowering immunity and liver function, commercialization of mushroom mycelium culture extracts, and adverse effects, and achieves no toxic effect on normal cells, and remarkable anti-cancer effect of low and medium

Inactive Publication Date: 2006-04-13
KIM JEONG +3
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides a method for producing a glycoside of isoflavone and β-D-glucan with anti-cancer and immunoenhancing effects using a mushroom mycelia culture. The method involves cultivating Agaricus blazei mycelia in a liquid medium containing isoflavone to produce a high molecular weight isoflavone-β-D-glucan, which is then separated from the liquid culture and purified. An autolysis enzyme is added to the high molecular weight isoflavone-β-D-glucan to produce a low and medium molecular weight isoflavone-β-D-glucan, which can be used as an anti-cancer and immunoenhancing agent. The use of liquid culture instead of solid culture allows for larger scale production at lower cost.

Problems solved by technology

Conventional anti-cancer agents have much toxicity to affect normal cells since they are lack of specificity to cancer cells, which results in a variety adverse effects such as depilation, lowering of immunity and liver function, etc.
The extracts of mushroom mycelium culture commercialized by this time have not been distributed on a large scale since the extraction process costs very high.
In general, the cultivation period of Lentinus edodes, Phellinus linteus, Ganoderma lucidum, etc. is long and the growth of mycelia is slow, which limits the amount of extracts.
However, the enzyme for the hydrolyzation is hard to obtain, which makes the cost high.
Further, the method of separating and purifying the medium or low molecular weight polysaccharides produced from the hydrolyzation has technical difficulty, so that the yield is very low.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Isoflavone-beta-D-glucan produced by Agaricus blazei in the submerged liquid culture and method of producing same
  • Isoflavone-beta-D-glucan produced by Agaricus blazei in the submerged liquid culture and method of producing same
  • Isoflavone-beta-D-glucan produced by Agaricus blazei in the submerged liquid culture and method of producing same

Examples

Experimental program
Comparison scheme
Effect test

example 1

Production and Separation of High Molecular Weight Isoflavone-β-D-glucan

[0072] The culture medium contained soybean powder or natural source including soybean powder. Natural isoflavone separated from soybean or synthetic isoflavone may also be used. The liquid medium were added with brown sugar as carbon source and inorganic salts, and then autoclaved at 120° C. for 30 minutes.

[0073] AB mycelia were inoculated in the liquid culture medium containing isoflavone and cultured with aeration or stirring for 1 to 7 days to produce high molecular weight isoflavone-β-D-glucan. The molecular weight of the high molecular weight isoflavone-β-D-glucan was at least 30,000.

[0074] Liquid culture of AB mycelia was extracted with boiling water at 100° C. for 10 hours, and the extract was autoclaved at 121° C. for 1 hour.

[0075] The extract of liquid culture of AB mycelia was treated with 80% ethanol to make precipitation and then centrifuged to remove supernatant. The precipitate was separated t...

example 2

Selection of Ethanol Fraction Having the Highest Content of High Molecular Weight Isoflavone-β-D-glucan

[0076] The extract of liquid culture of AB mycelia was suspended in ethanol to prepare 10 to 80% ethanol solution to induce precipitation. Each precipitate was separated and then applied to Biosep S-2000 column.

[0077]FIG. 2 is HPLC chromatograms of the extract of liquid culture of Agaricus blazei mycelia treated with various concentrations of ethanol solution (40, 50, 60, 70 and 80% ethanol) and then separated with Biosep S-2000 column. The chromatogram on the right side below represents the chromatogram of total extract of liquid culture of AB mycelia. In FIG. 2, horizontal axis represents time (minutes) and vertical axis represents length (mAU).

[0078] As shown in FIG. 2, the high molecular weight isoflavone-β-D-glucan, obtained by treating 80% ethanol to the liquid culture of AB mycelia to induce precipitation, hereinafter referred to ‘80 EP (80% ethanol precipitate)’, shows s...

example 3

Separation and Fractionation of Autolysis Enzyme

[0080] The separation of autolysis enzyme was carried out by filtrating the liquid culture of AB mycelia at reduced pressure. TCA (trichloroacetic acid) was added to the filtrate to the concentration of 10% and the mixture was placed at 4° C. for 24 hours. The resultant was centrifuged at 4° C. and 10,000 rpm for 15 minutes to obtain the precipitate.

[0081] The precipitate was further fractionated by DEAE (diethylaminoethyl) column chromatography and each fraction was determined its UV absorbance at 280 nm.

[0082]FIG. 3 shows fractionation of the precipitate of the liquid culture of Agaricus blazei mycelia by DEAE column chromatography. In FIG. 3, horizontal axis represents number of each fraction and vertical axis represents UV absorbance at 280 nm. As shown in FIG. 3, UV absorbance of each fraction was monitored and eight fractions were obtained showing UV absorbance at 280 nm. Fraction #2 (tube #8) showed the maximum UV absorbance....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
temperatureaaaaaaaaaa
pHaaaaaaaaaa
molecular weightaaaaaaaaaa
Login to View More

Abstract

The present invention relates to low and medium molecular weight isoflavone-β-D-glucan produced by submerged liquid culture of Agaricus blazei, a method of producing the isoflavone-β-D-glucan using autolysis enzyme of Agaricus blazei mycelia, and use of the isoflavone-β-D-glucan for anti-cancer and immunoenhancing effect.

Description

CROSS REFERENCE TO RELATED APPLICATION [0001] This application is a divisional application of U.S. patent application Ser. No. 10 / 890,537 filed Jul. 12, 2004, which claimed the priority of Korean Patent Application No. 2003-67439, filed Sep. 29, 2003, in the Korean Intellectual Property Office, the contents of which are incorporated by reference herein in their entirely.TECHNICAL FIELD [0002] The present invention relates to low and medium molecular weight isoflavone-β-D-glucan produced by submerged liquid culture of Agaricus blazei, a method of producing the isoflavone-β-D-glucan using autolysis enzyme of Agaricus blazei mycelia, and use of the isoflavone-β-D-glucan for anti-cancer and immunoenhancing effect. BACKGROUND ART [0003] Cancer holds the most fatal disease in the statistics of mortality, so that anti-cancer agents have been a matter of continued concern. Conventional anti-cancer agents have much toxicity to affect normal cells since they are lack of specificity to cancer ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C12P19/62A61K31/7048C12P19/44A61K31/716A61K35/74A61P35/00A61P37/04C08B37/00C12P19/04C12R1/645
CPCA61K31/7048A61P35/00A61P37/04C12P19/04
Inventor KIM, JEONG OKHA, YEONG LAEKIM, YOUNG SUKPARK, CHERL WOO
Owner KIM JEONG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com