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Secretome Profile-Facilitated In Vitro Fertilization

a secretome profile and in vitro fertilization technology, applied in the field can solve the problems of higher pregnancy rate, complicated and complex process, spontaneous abortion, etc., and achieve the effect of enhancing the pregnancy success rate of in vitro fertilization

Inactive Publication Date: 2011-10-06
FERTILITY LAB SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0015]The CDC data from 2006 shows that use of donor eggs in a 40 year old woman will result in a live birth 54% of the time. This compares to 20.6% using the woman's own eggs. Thus, an opportunity exists for the instrumentalities of this disclosure to facilitate success rates approximating the use of donor eggs when using one's own eggs, and may even permit higher success rates due to better selection of viable embryos.
[0016]In an embodiment, a system is provided for enhancing the pregnancy success rate of in vitro fertilization. The system includes an electronic system to configured to gather secretome data as a secretome profile of an embryo by quantitating proteins implicated in implantation success. A model is provided for use in recommending whether to implant the embryo on the basis of this secretome profile. The secretome date may be, for example, provided by use of mass spectroscopy or ELISA measurements.

Problems solved by technology

Due to expensive procedural costs, IVF is only attempted after the failure of less expensive fertility treatments.
Mammalian embryo implantation is a complex and intricate process involving numerous biological changes at both the embryo and endometrial level.
A blastocyst failing to implant or an endometrium failing to sustain growth and differentiation will result in spontaneous abortion.
Blastocyst stage transfers often result in higher pregnancy rates.
Despite progressively improving IVF pregnancy rates, the majority of transferred human embryos result in implantation failure.
Moreover, implantation success rates may decrease with the increasing maternal age, if donor eggs are not used.
However, embryo selection based on morphological qualities is not precise.
The transfer of multiple embryos, however, often results in multiple pregnancies, a major complication of IVF.
In general, multiple pregnancies, specifically, more than twins, hold maternal and fetal risks.
For example, multiple births are associated with increased risk of pregnancy loss, neonatal morbidity, obstetrical complications, and prematurity with potential for long term damage.
However, these limitations are not universally followed or accepted.

Method used

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Examples

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example 1

Secretome Profiling of Human Embryos Using Mass Spectrometry

[0040]The following nonlimiting example teaches by way of illustration, not by limitation, secretome profiling of a human embryo using mass spectrometry (MS). Human cleavage-stage embryos were cultured in 10 μL drops of G1 supplemented with 2.5 mg / mL recombinant albumin under oil at 37° C., 6% CO2, 5% O2 for 24 hours. The embryos were washed twice in G2 culture media and further cultured in 10 μL drops of G2 supplemented with 2.5 mg / mL recombinant albumin under oil at 37° C., 6% CO2, 5% O2 for 48 hours with a fresh drop of G2 media added after 24 hours. Spent media samples of blastocysts were transferred into 0.65 mL Eppendorf tubes. Control groups comprised media cultured and collected under the same conditions but without embryos.

[0041]Micro-drops of spent media were depleted of human serum albumin (HSA) using Cibracron Blue Activated SwellGel Discs (Themo Fisher Scientific, Rockford, Ill.). The proteins in the spent medi...

example 2

Secretome Profiling to Predict Implantation Potential of a Human Embryo

[0047]The following nonlimiting example teaches by way of illustration, not by limitation, the fabrication and employment of a customized immunoassay test kit for secretome profiling of a human embryo. Immunoassay test kit fabrication occurs by modifying the well surfaces of a 96-well microtiter plate. Each separate well of the microtiter plate is incubated with an unlabelled capture antibody that recognizes one specific protein from the secretome panel. Custom capture antibodies are purchased from Rockland Immunochemicals, Inc. After incubation for 12 hours at 4° C., capture antibodies passively adsorb to the well surface. Subsequently, all wells of the 96-well plate are washed three times with a buffer comprising 1× phosphate buffer saline and 0.1% Tween-20 (PBST), blocked with 1% bovine serum albumin (BSA) for 1 hour at ambient temperature and washed three times with PBST.

[0048]Human cleavage-stage embryos are...

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Abstract

Secretome profiling improves the pregnancy success rate of in vitro fertilization processes, while reducing the risk of multiple births.

Description

RELATED APPLICATIONS[0001]This application claims benefit of priority to U.S. Provisional Patent Application No. 61 / 321,448 filed Apr. 6, 2010, which is incorporated by reference to the same extent as though fully replicated herein.SEQUENCE LISTING[0002]This application includes a Sequence Listing, as set forth in an ASCII-compliant text file named “CCRMProtein_ST25.txt”, created on Apr. 6, 2011, and containing 2263 kilobytes, which is incorporated by reference to the same extent as though fully replicated herein.BACKGROUND[0003]1. Field of the Invention[0004]The invention relates to the field of in vitro fertilization (IVF), which is a process by which mammalian egg cells are fertilized by sperm outside the womb. More particularly, evaluation of a secretome profile is used to enhance the pregnancy success rate when the fertilized egg is implanted into a patient's uterus.[0005]2. Description of the Related Art[0006]IVF infertility treatment offers infertile couples a chance to have ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61B17/435C12M1/34
CPCG01N33/689G01N33/543G01N2800/52
Inventor SCHOOLCRAFT, WILLIAM B.KATZ-JAFFE, MANDYMCREYNOLDS, SUSANNA
Owner FERTILITY LAB SCI
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