Compositions of adult disc stem cells and methods for the treatment of degenerative disc disease

a technology for disc disease and stem cells, applied in the field of adult disc stem cells and methods for the treatment of degenerative disc disease, can solve the problems of morbidity, disability, and productivity loss, and no treatment modalities have served as the “magic bullet” to eliminate or consistently improve this condition, and the prosthesis ultimately fails

Inactive Publication Date: 2012-04-26
DISCGENICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0009]In one embodiment, the present invention provides a two dimen

Problems solved by technology

Back pain resulting from degenerative disc disease is a major cause of morbidity, disability, and lost productivity.
Despite the continued improvements in non-operative and operative treatment options for patients with lower back pain secondary to degenerative disc disease, no treatment modalities have served as the “magic bullet” to eliminate or consistently improve this condition.
Although initially there was a short period of symptom relief, the prosthesis ultimately failed secondary to subsidence of the implant within

Method used

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  • Compositions of adult disc stem cells and methods for the treatment of degenerative disc disease
  • Compositions of adult disc stem cells and methods for the treatment of degenerative disc disease
  • Compositions of adult disc stem cells and methods for the treatment of degenerative disc disease

Examples

Experimental program
Comparison scheme
Effect test

example 1

A Method of Growing Discospheres

[0183]A biopsy specimen of human nucleus pulposus was minced into pieces approximately 2-3 millimeters in size and transferred to a 50 ml falcon tube containing 30 ml of Phosphate buffered saline (PBS) supplemented with standard antibiotics and antimycotics (standard penicillin / streptomycin solution (GIBCO BRL) in concentration 1:100).

[0184]PBS was aspirated and 30 ml of Dulbecco's Modified Eagle Media with F12 (DMEM / F12) medium containing 300 U / ml of Collagenase II solution was added to the 50 ml tube.

[0185]The tube was placed in a horizontal position in a shaker incubator at 37° C. at 100 RPM for 2-3 hours until fragments were completely dissociated.

[0186]The cell suspension was filtered through a nylon mesh into a 50 ml falcon tube and triturated with a fire-polished pasteur pipette to form a single-cell suspension. A cell count was performed at this point to determine the cell concentration.

[0187]The cell suspension was then centrifuged at room te...

example 2

A Method of Expanding Discospheres Cell Culture

[0194]Discospheres obtained by the method disclosed in Example 1 were dissociated by incubation at 37° C. in DMEM / F12 medium supplemented by collagenase II (300 U / ml).

[0195]Dissociated cells were expanded in 6-well plates according by passaging the cells using the same plating and culture techniques as described in Example 1.

example 3

A Method of Obtaining a Spinal Disc Collagen Scaffold (Annulus)

[0196]A postmortem (rabbit cadaver) intervertebral disc was removed by dissection with the vertebral endplates left intact. The intervertebral disc sample was soaked in 4 M guanidine thyocyonate for 24 hours at room temperature to remove intradisc biomaterial. After 24 hours, the intradisc biomaterial was liquidfied.

[0197]The liquid was aspirated, and the remaining disc scaffold was washed 3 times with room temperature PBS.

[0198]At this stage the disc scaffold can be stored in PBS at 4° C. up to one year.

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PUM

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Abstract

This invention provides a tissue growth apparatus comprising one or more discospheres and a method of producing nucleus pulposus cells comprising the step of growing one or more discospheres on the tissue growth apparatus. This invention also provides a neo-engineered disc comprising nucleus pulposus cells, and related methods of production and methods of use.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims the benefit of U.S. Provisional Application Ser. No. 61 / 442,315, filed Feb. 14, 2011, and is a continuation-in-part of U.S. application Ser. No. 12 / 216,544 filed Jul. 7, 2008, which claims the benefit of U.S. Provisional Application Ser. No. 60 / 929,792, filed Jul. 12, 2007 which are hereby incorporated by reference in their entirety.FIELD OF INVENTION[0002]This invention provides a tissue growth apparatus comprising one or more discospheres and a method of producing nucleus pulposus cells comprising the step of growing one or more discospheres on the tissue growth apparatus. This invention also provides a neo-engineered disc comprising nucleus pulposus cells, and related methods of production and methods of use.BACKGROUND OF THE INVENTION[0003]Back pain resulting from degenerative disc disease is a major cause of morbidity, disability, and lost productivity. Back pain is the most frequent cause of activity limitati...

Claims

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Application Information

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IPC IPC(8): C12N5/077
CPCA61K35/12C12N5/0655C12N2500/25C12N2500/46C12N2533/78C12N2501/11C12N2501/115C12N2501/392C12N2533/54C12N2500/90A61K35/32C12N5/066C12N5/0662C12N2500/84
Inventor DUNTSCH, CHRISTOPHERKUKEKOV, VALERYIGNATOVA, TATYANA
Owner DISCGENICS
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