Method for Producing Glucosamine by Culturing Microorganism with Low-cost Medium
a microorganism and low-cost technology, applied in the direction of fertilization, etc., can solve the problems of affecting the purity of glucosamine, affecting the metabolism of cells in joints, and lowering the glucosamine in the human body, so as to reduce the cost of the medium
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example 1
Test Medium
1. Experimental Strains
[0026]The invention discloses a method for producing glucosamine by fermenting a non-gene-transferred microorganism with changing medium. The microorganism used was one of the following: Monascus pilosus BCRC31527, with an accession number corresponding to ATCC 22080, and Aspergillus sp. BCRC31742, with an accession number corresponding to UPCC 3868. Both of the above-mentioned strains were purchased from Food Industry Development and Research Institute, Hsin-chu, Taiwan, ROC.
2. Medium
[0027]An appropriate medium was selected based the different characteristics of the above-mentioned two strains for carrying out the production of glucosamine. Media selected for each of the two strains were listed in Table 1.
TABLE 1Media for various glucosamine-producing strainsComponentsConcentrationStrainMediumin medium(g / L)MonascusRBA (pH 5)Rice bran25pilosusB-grade white25BCRC31527crystal sugarNH4Cl15Aspergillus sp.WP1 (pH 7)Superior white fineBCRC31742granulated ...
example 2
Shaking-Flask Fermentation Test
[0036]Various strains described in Example 1 was activated separately through three region streak plate culturing in PDA solid medium (Potato Dextrose Agar: 200 g / L Diced potatoes, 20 g / L Glucose, 15 g / L Agar) at 30° C. for 5 days. Then, single colony was picked up and placed in 200 cm3 sterilized PDB liquid medium (Potato Dextrose Broth: 20 g / L Diced potatoes, 4 g / L Glucose) contained in a 250-cm3 shaking-flask, followed by secondary activation through incubating in a thermostatic incubator at 30° C. and 200 rpm for 7 days.
[0037]Stains thus-activated was used to inoculate in a suitable medium (Table 1), and the pH of the medium was controlled as followed: pH of the medium used for microorganism Monascus pilosus BCRC 31527 was pH 5, pH of the medium used for microorganism Aspergillus sp. BCRC 31742 was pH 7. Thereafter they were incubated at a temperature of 30° C., and 200 rpm for 7 days. Samples were taken and the cell dry weight and yield of glucosa...
example 3
Fermentation Test in Fermenter
[0047]In this example, at first, Aspergillus sp. BCRC 31742 described in Example 1 was subjected to secondary activation culturing in accordance with the manner as in Example 2. Then, a fermentation test was carried out in a batchwise stirring fermenter as described below. The 100 cm3 thus-activated Aspergillus sp. BCRC 31742 liquor was inoculated in WP1 medium wothin the fermenter, and with a operation volume of 2 dm3, its was subjected to fermentation culture under optimal conditions obtained from shaking-flask experiment: pH 7, a temperature of 30° C., a rotational speed of 200 rpm, and with pure oxygen introduced through external lines to control the dissolved oxygen concentration at 10%.
[0048]After recovered from the fermenter, the fermentation liquor was filtered with suction to yield bacterial cake. Next, a sample of the bacteria cake was dried at 100° C., and the ratio of wet cell weight to dry cell weight was determined. Separately, wet cells w...
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