Microorganism for expressing a human membrane protein

a technology of membrane protein and microorganism, which is applied in the field of isolated genetically modified living non-mammal organisms, can solve the problems of heterologous expression of membrane protein from mammal cells in wild type strains, difficulty in expressing sert,
US20120156695A1Inactive Publication Date: 2012-06-21NOVOZYMES AS
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
NOVOZYMES AS
Publication Date
2012-06-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an isolated, genetically modified, living non-mammal organism, having increased HMG-CoA-reductase activity compared to the wild type, and having reduced C24-methyltransferase and / or delta22-desaturase activity compared to the wild type. The invention is characterized in that the organism has increased dehydrocholesterol-delta70-reductase activity compared to the wild type. The invention further relates to different uses of such an organism, to a test kit comprising such an organism, and to a membrane extract of such an organism.
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Description

FIELD OF THE INVENTION

[0001] The invention relates to an isolated genetically modified living non-mammal organism, having increased HMG-CoA reductase activity compared to the wild type, and having a reduced C24 methyltransferase and / or delta22 desaturase activity compared to the wild type, to the uses thereof, a nucleic acid construct for the production thereof, a kit containing the organism, and a membrane extract of such an organism.PRIOR ART AND BACKGROUND OF THE INVENTION

[0002] An organism of the structure mentioned above is known from the document WO 03 / 064650. This organism is suitable for the biosynthesis of 7-dehydrocholesterol. The motivation for creating this organism is based on the need to produce precursor products to vitamin D3, in particular 7-dehydrocholesterol, in large amounts and under economical conditions.

[0003] In a completely different context, there is a need of membrane proteins, in particular human membrane proteins, functionally in a native conformation in at...

Claims

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