Compositions and methods for bamboo pulping
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example 1
Phyllostachys bissetti
[0388]Starting with a bamboo plant between the ages of 3 months and 3 years, a node from the cane with the lateral shoot just breaking the sheath was used as the explant. Each nodal section was cut into 3-5 millimeter sections with the shoot intact. The outer sheaths were peeled off and discarded and the remaining nodal section piece put into a 10% bleach solution with a final concentration of 0.6% sodium hydrochloride. The explant in bleach solution was placed onto a Lab Rotators, Adjustable speed, Barnstead / Lab line orbital Shaker (model number KS 260) shaker table for 1 hour at 6-9 revolutions per minute. The explants were then put into a 1% bleach solution with a final concentration of 0.06% sodium hydrochloride, and placed back onto the shaker table for 30 minutes. This 1% bleach solution step was then repeated.
[0389]Individual explants were then placed on a Stage 1 media (15-25 mL) within a tube and the tubes were placed into a regulated clean growth cha...
example 2
Fargesia denudata
[0392]In the example of Fargeria denudata, the explants were chosen and disinfected as in Example 1. The explants were then transferred into jars containing a Stage 1 media, in this Example, standard b-12c-iv (liquid; 30-40 mL) as described in Example 1 but for the use of jars. Explants were taken off the media after the third cycle if multiplication was occurring. If multiplication was not occurring or not occurring to a significant degree, explants were left on the media for a fourth cycle. Contaminated tubes were discarded.
[0393]The cultures were then transferred onto a Stage 2 media, in this Example, standard b-11-iv (liquid) in jars on a rotating shelf that provides 6-9 revolutions per minute. The cultures remained on standard b-11-iv media at a pH of 5.7 for 10-120 day cycles (usually 14 day cycles) until the desired number of shoots was obtained by separation into new jars and further expansion. One-fifteen shoots per jar were obtained per multiplication cyc...
example 3
Pleioblastus fortunei
[0394]In the example of Pleioblastus fortunei, the explants were chosen and disinfected as in Example 1. The explants were then transferred into tubes containing a Stage 1 media, in this Example, standard b-12c-iv also as described in Example 1. Shoots were then transferred to a Stage 2 media, in this Example, standard b-9-iv in magenta boxes (40-50 mL). They remained on standard b-9-iv media for 10-120 day cycles (usually 14 day cycles) until the desired number of shoots was obtained by separation into new boxes and further expansion. One-twenty shoots per box were obtained per multiplication cycle. The shoots were then placed on a Stage 3 media, in this Example, standard BR-2-iv for 10-120 days (usually 14-21 days).
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