Compositions and Methods for Implantation of Processed Adipose Tissue and Processed Adipose Tissue Products

Abstract

The invention provides compositions and methods for the preparation of processed adipose tissue. The invention further provides methods of use of the processed adipose tissue.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority to Provisional Patent Application Ser. No. 61 / 232,915 filed on Aug. 11, 2009. This application is related to U.S. Provisional Patent Application Ser. No. 61 / 065,322 filed on Feb. 11, 2008 and PCT application PCT / US2009 / 00887 filed on Feb. 11, 2009 and published on Aug. 20, 2009 as WO 2009 / 102452. The applications are all incorporated herein by reference in their entirety.BACKGROUND[0002]The field of regenerative medicine aims to provide tissue substitutes for reconstruction secondary to trauma, disease or congenital abnormalities. While biomaterials and cells are often employed to regenerate new tissues, these methods tend to be costly and require significant time for new tissue formation. Restoration of soft tissue form is critical for a number of applications including trauma reconstruction, breast reconstruction, and cosmetics (nasolabial folds, wrinkles, etc). In general there are two approaches today;...

AI Technical Summary

Benefits of technology

[0104]As used herein, a “substantial immune response” is understood as an immunological response of a subject after implantation of a processed adipose tissue of the invention that requires intervention by a medical professional (e.g., need for removal of the processed adipose tissue, administration of immunosuppressive drugs); or results in substantially reduced lifetime of the implanted processed adipose tissue, for example the duration of the implanted processed adipose tissue is decreased by 50% or more, at least 60% or more, at least 70% or more, at least 80% or more, or at least 90% or more, and wherein the implanted processed adipose tissue is infiltrated with at least one type of inflammatory cell including, but not limited to, macrophages, neutrophils, and eosinophils. A substantial immune response does not include temporary and transient (e.g., one week or less, 6 days or less, 5 days or less, 4 days or less, 3 days or less, 2 days or less, or one day or less) of general redness, irritation, and swelling at the site of implantation that is reduced over time, and may not be a response to the implanted tissue per se, but instead to the disruption of the skin or otherwise adjacent tissue due to the disruption or stretching of the skin or tissue associated with implantation.

Problems solved by technology

While biomaterials and cells are often employed to regenerate new tissues, these methods tend to be costly and require significant time for new tissue formation.

However, such procedures also have their limitations.

Such flaps are necessarily limited in size by the amount of tissue present in the woman for use, and transfer of muscle from the abdomen or back can extend recovery time and result in donor site morbidity.

Such loss of persistence requires multiple procedures to maintain the desired correction.

In addition, implanted adipose tissue often leads to post-operative calcifications.

This phenomena is of particular importance for women with a history of breast cancer undergoing breast reconstruction following mastectomy, as the calcifications may interfere with mammography readings and result in multiple, unnecessary breast biopsies and anxiety.

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