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Eukaryotic cell display systems

a cell display and eukaryotic technology, applied in the field of protein display, can solve the problems that prokaryotic host cells are typically not able to accomplish the full range of post-translational modifications, and the eukaryotic proteins cannot be functionally expressed in prokaryotic cells, and achieve the effect of facilitating direct expression of library proteins

Inactive Publication Date: 2012-12-27
MERCK SHARP & DOHME CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0016]Co-expression of an expression vector of the invention in combination with a corresponding helper vector directs the display of the polypeptide product of the display library members anchored to the cell membrane or cell wall of the host cell. Surface display results from the pairwise interaction of the adapters (i.e., adapter1 fused to the protein product of the display library, and adapter2 fused to a host cell specific anchor protein) which has the effect of directing the display of the protein library on the surface of the host cell.
[0018]The use of an expression vector of the invention in the absence of a helper vector, results in the expression of the encoded proteins as soluble proteins by the host cells. Therefore, the disclosed display vectors also facilitate the direct expression of library proteins as either soluble protein or as intracellular proteins without any molecular manipulation (i.e., DNA digestion and / or ligation) of the vector. Accordingly, the invention also provides an efficient method to evaluate the functional characteristics of the members of the display library proteins.

Problems solved by technology

However, despite the successful use of phage display in antibody discovery and engineering protocols, there are a number of drawbacks associated with the expression and display of eukaryotic proteins in prokaryotic systems.
For example, some eukaryotic proteins cannot be functionally expressed in prokaryotic cells.
In addition, prokaryotic host cells are typically not able to accomplish the full range of post-translational modifications that are characteristic of eukaryotic host cells.
There are a number of drawbacks associated with the use of a cell surface display system based on fusing the protein library to a cell surface anchor protein.

Method used

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Examples

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example 1

Construction Yeast Expression Vector pMAT9

[0107]pMAT9 vector (FIG. 2A) comprise a expression cassette for an anti-VEGF scFv antibody fused with adapter1 (GR1) (SEQ ID NO:1) and detectable HA and His6 tag (DH tag). It was constructed by insertion of a fully synthetic gene fragment into a commercial pESC-TRP vector (Stratagene) through cloning sites EcoRT and Pad. The scFv-GR1 fusion protein is under the control of a galactose-induced promoter GAL10 and a signal sequence of yeast endo-beta-1,3-glucanase (Bg12). The fusion gene sequence (SEQ ID NO:3) was confirmed by standard DNA sequencing method.

example 2

Construction Yeast Expression Vector pMAT12

[0108]pMAT12 (SEQ ID NO: 4) provides an expression vectors that is suitable for expression in yeast cells. As shown in FIG. 2B, pMAT12 is created on the backbone of commercial vector pUC 19 by insertion at Aatll and Pcil sites with a fully synthetic DNA fragment (Codon Devices). This fully synthetic DNA fragment comprises (1) f1 ori; (2) a expression cassette for the adapter GR1 (SEQ ID NO:1) fusion, which is driven by a yeast pGAL1 promoter. The sequence of anti-VEGF scFv antibody is built in the downstream of a yeast signal sequence (yeast endo-B-1,3-glucanase protein Bg12p). The HA-His6 tag (DH-tag) sequences are upstream of GR1 sequence for protein detection and Ni-NTA purification. (3) yeast CEN / ARS ori for replication; and (4) a expression cassette for yeast TRP1 auxotrophic marker. The synthetic DNA sequence was confirmed by standard DNA sequencing method.

example 3

Yeast Helper Vector pMAT7

[0109]pMAT7, which is graphically depicted in FIG. 3A, is another yeast display helper vector which expresses a fusion protein comprising the yeast outer surface protein Aga2 in frame with adapter 2 (GR2) (SEQ ID NO: 2). This vector was created from the yeast helper vector pMAT3 by replacing BamHI-HindIII fragment with a synthetic DNA fragment of 208 bp. This synthetic DNA comprises the sequence encoding yeast out well protein Aga2. Using BioFab platform technology of Codon Devices, the errors generated from oligo synthesis were corrected by oligo selection with sequences complementary to the synthetic genes, and affinity purification of Mut-S protein column. The nucleotide sequence of pMAT7 (SEQ ID NO: 5) was confirmed by standard DNA sequencing.

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Abstract

The present invention provides expression vectors and helper display vectors which can be used in various combinations as vector sets for display of polypeptides on the outer surface of eukaryotic host cells. The expression vector of the invention can be used alone for soluble expression without having to change or reengineer the display vectors. The display systems of the invention are particularly useful for displaying a genetically diverse repertoire or library of polypeptides on the surface of yeast cells, and mammalian cells.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a of U.S. application under 37 CFR 1.53(b) which claims the benefit of U.S. Provisional Application No. 61 / 003,413 filed Nov. 16, 2007.TECHNICAL FIELD OF THE INVENTION[0002]This invention relates to the field of protein display and provides display systems which facilitate the display of protein libraries on the surface of eukaryotic host cells, including yeast cells and mammalian cells. The compositions and methods of the invention are particularly useful for identifying proteins with desired properties from a vast repertoire of proteins. This system also provides methods for producing soluble protein for use in functional assays and for directing expressed proteins to different cellular organelles without any molecular manipulation of the display vector.BACKGROUND OF THE INVENTION[0003]Phage display systems are regarded as a core technology platform for the construction and screening of polypeptide libraries, particu...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C40B50/06C12N15/63C12N1/19C12N5/10
CPCC40B40/02C12N15/1037
Inventor WANG, KEVIN CAILILUO, PETER PEIZHIZHONG, PINGYUWANG, JIAN
Owner MERCK SHARP & DOHME CORP
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