Method for the preparation of a heat stable oxygen carrier-containing composition facilating beta-beta cross-linking
a technology of oxygen carrier and composition, which is applied in the field of preparation of heat stable oxygen carrier-containing pharmaceutical composition, can solve the problems of affecting the sustainability of functional hemoglobin in circulation, affecting the stability of plasma hemoglobin, and substantial renal injury
Image
Examples
example 1
[0061]Process Overview
[0062]A schematic flow diagram of the process of the present invention is illustrated in FIG. 1. Bovine whole blood is collected into an enclosed sterile container / bag containing 3.8% (w / v) tri-sodium citrate solution as anti-coagulant. Blood is then immediately mixed well with tri-sodium citrate solution to inhibit blood clotting. Red blood cells (RBC) are isolated and collected from plasma and other smaller blood cells by an apheresis mechanism. A “cell washer” is used for this procedure with gamma sterilized disposable centrifuge bowl. RBC are washed with an equal volume of 0.9% (w / v sodium chloride) saline.
[0063]Washed RBC are lysed to release hemoglobin content by manipulating hypotonic shock to the RBC cell membrane. A specialized instant cytolysis apparatus for RBC lysis device depicted in FIG. 2 is used for this purpose. Following RBC lysis, hemoglobin molecules are isolated from other proteins by tangential-flow ultrafiltration using a 100 kDa membrane...
example 2
[0065]Time & Controlled Hypotonic Lysis and Filtration
[0066]Bovine whole blood is freshly collected and transported under a cool condition (2 to 10° C.). The red blood cells are separated from the plasma via a cell washer and subsequently with a 0.65 μm filtration. After washing the red blood cells (RBC) filtrate with 0.9% saline, the filtrate is disrupted by hypotonic lysis. The hypotonic lysis is performed by using the instant cytolysis apparatus depicted in FIG. 2. The instant cytolysis apparatus includes a static mixer to assist in cell lysis. A RBC suspension with controlled hemoglobin concentration (12-14 g / dL) is mixed with 4 volumes of purified water to generate a hypotonic shock to RBC cell membranes. The period of hypotonic shock is controlled to avoid unwanted lysis of white blood cells and platelets. The hypotonic solution passes through the static mixer portion of the instant cytolysis apparatus for 2 to 30 seconds or otherwise a time sufficient to lyse the red blood ce...
example 3
[0068]Viral Clearance Study on Stroma-Free Hemoglobin Solution
[0069]In order to demonstrate the safety of the product from this invention, the virus removal abilities of (1) 0.65 μm diafiltration step and (2) 100 kDa ultrafiltration step are demonstrated by virus validation study. This is done by the deliberate spiking of a down-scaled version of these two processes with different model viruses (encephalomyocarditis virus, pseudorabies virus, bovine viral diarrhea virus and bovine parvovirus). In this study, four types of viruses (see Table 3) are used. These viruses vary in their biophysical and structural features and they display a variation in resistance to physical and chemical agents or treatments.
TABLE 3TargetSizeVirusModel VirusTaxonomyGenomeStructure[nm]Stability*HepatitisBovine viral diarrheaFlaviviridaessRNAenveloped40-60lowC virusvirus (BVDV)—EncephalomyocarditisPicornavirusssRNAnon-25-30mediumvirus (EMCV)envelopedParvovirusBovine parvovirusParvoviridaessDNAnon-18-26very...
PUM
| Property | Measurement | Unit |
|---|---|---|
| temperature | aaaaa | aaaaa |
| molecular weight | aaaaa | aaaaa |
| temperature | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- IPC
- A61K9/00; C12N5/071; A61P9/00; A61K38/42
- CPC
- A61K38/42; A61P17/00; A61P43/00; A61P7/00; A61P7/08; A61P9/00; A61K47/50; A61K9/08
- Inventors
- WONG, BING LOU; KWOK, SUI YI