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Composition comprising the extract of herbs for preventing or treating neurodegenerative disorders

a technology for neurodegenerative disorders and herbal extracts, applied in the direction of biocide, plant/algae/fungi/lichens, drug compositions, etc., can solve the problems of insignificant regeneration and improvement of motor neuron and its function, difficult diagnosis, and significant improvement, etc., to promote the formation of neurites, increase the amount of nerve growth factor, and increase the number of neural cell proliferation

Inactive Publication Date: 2014-02-13
DONG A ST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent describes a herb extract made from a combination of two herbs, Dioscorea Rhizoma and Dioscorea nipponica, that can increase the amount of nerve growth factor (NGF) in the body, stimulate the growth of neural cells, and improve cognitive abilities. This herb extract can be used for the prevention or improvement of neurodegenerative disorders.

Problems solved by technology

However, the diagnosis in this case is difficult to make because it shows multiple symptoms and the varied diseases have the common clinical signs.
As a result, the regeneration and improvement of motor neuron and its function was insignificant.
In case of the experiment which attempted to treat the patient having Alzheimer's disease using NGF, it couldn't show the significant result since there were side effects, the limit of injecting amount, the drug delivery and uncertain pharmacodynamics.

Method used

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  • Composition comprising the extract of herbs for preventing or treating neurodegenerative disorders

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0042]Preparation of the Mixed Herb Extracts of Dioscorea Rhizoma and Dioscorea nipponica.

[0043]Dioscorea Rhizoma and Dioscorea nipponica, both in a dry condition, were purchased from a herb medicine shop in Kyoungdong market, Korea. After impurities were removed therefrom, the herbs were chopped with a cutter and mixed at a weight ratio of 3.5:1 Dioscorea Rhizoma:Dioscorea nipponica. To 2 kg of the mixture was added 10 L of a 50% ethanol solution, followed by incubation at room temperature for 48 hours with stirring. The herb mixture was removed by filtration, and the filtrate was concentrated in a vacuum and freeze dried to afford a mixed herb extract (crude extract) (see Table 1).

TABLE 1Yield of the mixed herb extractsAmountD.D.Amount ofExtractionExtractionResultYieldRhizomanipponicaSolventsolventwashtemperaturetime(g)(%)Example 11.55 kg0.45 kg50% EtOH10 L1 LRoom2 days212.8510.64Temperature

experimental example 1

[0051]Effect of the Herb Extracts on Increasing the Secretion of Nerve Growth Factor

[0052]The effects on increasing and enhancing the secretion of NGF by Example 1 was identified by using C6 glioma, a cell strain of neuroglioma of rats producing NGF. Comparative examples 1 to 10 were the control groups of this experiment.

[0053]C6 cell was cultured in 24-well cell culture plate with medium, DMEM (adding 5% FBS, 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyriuvate, 50 μg streptomycin, 100 U / ml penicillin) in 37° C. and 5% of CO2. After adjusting the cultured cell in the 2×105 cell / well concentrations, Example 1, Comparative examples 1 to 10 were treated by 100, 250, and 500 μg / ml. In the same culture condition, the cell was cultured for 2 days and then the concentration of NGF in the medium was measured by using ELISA. (See Table 5)

TABLE 5Effects on increasing the secretion of NGFD. Rhizoma:D. nipponicaConcentrationcompared to the totalConcentrationof NGFweight(μg / ml)(% of control)Exa...

experimental example 2

[0059]Effect of the Herb Extracts on Improving Cognitive Abilities

[0060]ICR mice (male, 6 age of the weeks, 25-28 g) provided from Daehan Biolink (Chungbuk, Korea) were using after 7 days of being raised and adapted in a clean cage of college of pharmacy in Kyunghee university. They were allowed to have feeds (Daehan Biolink, Chungbuk, Korea) and water freely. The temperature (22±2° C.), humidity (53±3%) and cycle of light and dark (12 hours) were controlled automatically.

[0061]10 mice were assigned to each group. Saline was orally administered to mice in the control group in 5 mL per kg of weight. 10, 100 mg / kg of Example 1, Comparative examples 1 to 10 respectively melted in saline solution were orally administered to mice in the treatment group. Oral administration was done once a day and 1 hour after the last oral administration, passive avoidance test were conducted as follows.

[0062]Passive avoidance test was conducted in the A modified shuttle with two communicating (7×7 cm sl...

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PUM

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Abstract

This invention relates to a pharmaceutical composition and a health functional food for preventing or improving neurodegenerative disorders comprising mixed herb extracts of Dioscorea Rhizoma and Dioscorea nipponica in a weight ratio of 3.5:1 (w / w). The herb extracts mixed Dioscorea Rhizoma and Dioscorea nipponica in a weight ratio of 3.5:1 have the synergetic effects on increasing the amount of nerve growth factor in vivo, increasing the neural cell proliferation, promoting the formation of neuritis and enhancing cognitive abilities. Thus, the herb extracts of the present invention may be used for a pharmaceutical composition and a health functional food for preventing or treating neurodegenerative disorders.

Description

TECHNICAL FIELD[0001]This invention relates to a composition comprising the extract of herbs for preventing or treating neurodegenerative disorders, wherein it comprises Dioscorea Rhizoma and Dioscorea nipponica mixed in a weight ratio of 3.5:1 (w / w).ACKNOWLEDGEMENT[0002]This work was supported by the Global Leading Technology Program of the Office of Strategic R&D Planning (OSP) funded by the Ministry of Knowledge Economy, Republic of Korea. (No. 10039303)BACKGROUND ART[0003]Neurodegenerative disorders mean a gradually structural and functional loss of a nerve cell (neuron). They usually affect a particular part of the nervous system, accompanying the symptoms such as dementia, extrapyramidal disease, cerebellar disorder, dysesthesia or dyskinesia. The complex symptoms may be shown when affecting the various parts at the same time. A diagnosis is gotten by a patient's clinical sign. However, the diagnosis in this case is difficult to make because it shows multiple symptoms and the ...

Claims

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Application Information

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IPC IPC(8): A61K36/8945
CPCA61K36/8945A61P25/00A61P25/14A61P25/16A61P25/28A23L2/52A23V2002/00A61K9/0019A61K9/08A61K9/20A61K9/48A61K2236/15A61K2236/333A61K2236/51A61K2236/53A23L33/105
Inventor KIM, SOON-HOESON, MI-WONCHOI, SANG-ZINKIM, HYE-JURYU, JA-YOUNGKIM, SUN-YEOU
Owner DONG A ST CO LTD
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