Polymer dot compositions and related methods
a technology of polymer dots and compositions, applied in the field of polymer dots, can solve the problems of low brightness, insufficient phototability, and limitations of dot compositions
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example 1
[0047]This example describes an example lyophilization, a freeze-drying / dehydration technique, that can be used to prepare Pdot bioconjugates for long-term storage or shipping, provided the right conditions are used. Lyophilization is an important practical advance for making Pdots practical to use in biomedical research.
[0048]Materials. Poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(1,4-benzo-(2,10,3)-thiadiazole)] (PFBT; MW, 157 000 Da; polydispersity, 3.0), Poly(9,9-dioctylfluorenyl-2,7-diyl) end capped with dimethyl phenyl (PFO, MW 120000 Da), poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(1,4-benzo-(2,1′,3)-thiadiazole)]10% benzothiadiazole (PF10BT, MW 100000 Da) and poly[2-methoxy-5-(2-ethylhexyloxy)-1,4-(1-cyanovinylene-1,4-phenylene)] (CNPPV, MW 15000 Da) were purchased from American Dye Source Inc (Quebec, Canada). PFBT directly functionalized with carboxylic acid (PFBT-COOH) groups was synthesized in our lab. Polystyrene-grafted ethylene oxide functionalized with carboxyl groups (PS-P...
example 2
[0072]This example describes the effect of sucrose concentration on the lyophilization of Pdots. We used a series of sucrose concentrations (0%, 1%, 10%, 20%, 50%) (w / v) to lyophilize Pdot. Two types of Pdots were used. One is PFBT (Mw=73 k)+30% (w / w) PS-PEG-COOH, and the other is the directly functionalized PFBT-COOH 2%. The Pdots were prepared using nanoprecipitation as described in Example 1. The samples had a size of 21 nm at 20 nM concentration in aqueous solution. Different concentration of sucrose was added to the Pdot aqueous solution. The sample was then lyophilized. After lyophilization, the sample was stored in −80° C. freezer for 1 day and then re-dispersed in aqueous solution.
[0073]Size and quantum yield were measured to describe whether there is any change of the Pdot after lyophilization (FIG. 8). Without the addition of sucrose (0%), a size as large as 220 nm for lyophilized PFBT / PS-PEG-COOH Pdot and 230 nm for lyophilized PFBT-COOH2% (their unlyophilized counterpart...
example 3
[0074]This example describes the use of several lyophilization agents in the application of Pdot lyophilization. The direct functionalized PFBT-COOH 2% Pdots and BODIPY-690 were prepared using nanoprecipitation method as described in Example 1. Both the as-prepared Pdots had a size of 20 nm and at a concentration of 20 nM in aqueous solution. Different lyophilization agents with 5-20% (w / v) were added to the Pdot aqueous solution. In the combination of lyophilization agents, the total agents concentration was 10% (w / v) in the solution. The sample with lyophilization agent was then lyophilized. After lyophilization, the sample was stored in −80° C. freezer for 1 day and then re-dispersed to aqueous solution.
[0075]FIG. 9 in the upper panel shows the chemical structures of the lyophilization agents used for the Pdots; they are sucrose, glucose, mannitol, trehalose, maltose, hydroxypropyl-cyclodextrin, and bovine serum albumin (BSA). In addition, two combination agents were used; they a...
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