Regulation of apoptosis in aquatic organisms by aquabirnavirus
a technology of aquabirnavirus and apoptosis, which is applied in the direction of biocide, peptide/protein ingredients, antibody medical ingredients, etc., can solve the problems of not providing any insights which delineate the apoptosis process, and achieve the rescue or delay of the apoptosis cell death process, prevent the induction of dna, and maintain the expression level of mcl-1
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Image
Examples
embodiment 1
orphological Changes During Apoptosis
[0061]Green fluorescent protein (GFP) from the jellyfish Aequorea victoria is a revolutionary report molecule for monitoring gene expression and fusion protein localization in vivo or in situ and in real time. One of the most useful aspects of GFP for biological studies is that it can be monitored in living cells. In the present study, a variant type of GFP (EGFP) serves as a marker for visualizing the dynamic apoptotic cell morphological changes and for tracing membrane integrity changes during apoptosis by fluorescence microscopy, as well as for quantitation of the intra- and extracellular release of EGFP during apoptosis by western blotting and fluorometry.
[0062]The use of EGFP to study the apoptotic process is illustrated in the following experimental designs, results, and discussion:
(A) Experimental Designs
(1) Wild-Type CHSE-214 Cells, CHSE-214-EGFP Cells, and Viruses
[0063]Chinook salmon embryo cells (CHSE-214) were obtained from American Ty...
embodiment 2
ell Death Via An Mcl-1 Dependent Pathway
[0084]Mcl-1 belongs to the Bcl-2 family, which is known as the “apoptosis-inhibiting protein”. The founding member of this family is the bcl-2 protooncogene which was initially isolated from a follicular lymphoma (Bakhshi et al. (1985), Cell 41:889–906). Mcl-1 was originally identified from the differentiating human myeloid leukemia cell line ML-1. Its expression was found to increase early in the induction or “programming” of differentiation of ML-1 cells before the appearance of differentiation markers. The coding region of mcl-1 was sequenced and found to have a pronounced region of sequence homology to bcl-2 in the carboxyl-terminal region (Kozopas et al. (1993), supra). Unlike bcl-2, mcl-1 contains a strong PEST sequence (enriched in proline, glutamic acid, serine and threonine) which is present in a variety of proteins that undergo rapid turnover. Overexpression of exogenously introduced mcl-1 has been shown to cause a prolongation of vi...
PUM
Property | Measurement | Unit |
---|---|---|
Fluorescence | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com