The invention belongs to the technical field of a communication system, and specifically relates to a CAN bus accurate timing and assembly line testing communication system and method which can achieve high-reliability, high-precision, assembly line type and hot plug type communication with a servo driver. The system comprises an actuator A, an actuator B, an actuator C, a driver A, a driver B, a driver C, a ground control power supply module A, a CAN bus communication interface card, a CAN bus timing communication system, a ground power power-supply module B and a CAN bus. The ground power power-supply module B is connected with the actuator A, the actuator B and the actuator C. The ground control power supply module A is connected with the driver A, the driver B and the driver C. The CAN bus is connected with the driver A, the driver B and the driver C. The actuator A is connected with the driver C, the actuator B is connected with the driver B, and the actuator C is connected with the driver A.