The invention relates to a sampling device, in particular to a layered quantitative sampling device for petroleum detection. The invention provides the layered quantitative sampling device for petroleum detection, which can be used for quantitatively sampling petroleum. The layered quantitative sampling device for petroleum detection comprises a fixing frame, a first connecting plate, connecting frames, a fixing plate, a fixing rod, a first telescopic rod, a second connecting plate, a pulley, a pull rope, a first spring, a third connecting plate, a first connecting block, a first rotating shaft, a loading frame, a shell, a lifting mechanism and a rotating mechanism, wherein the first connecting plate is arranged on the inner side of the middle part of the fixing frame, and the connecting frames are installed on the left and right sides of the middle part of the fixing frame. Through the lifting mechanism, a worker can move the loading frame to a petroleum sampling position only by rotating a rocker to sample petroleum, the sampling amount of the petroleum can be controlled through the rotating mechanism, and excessive petroleum is prevented from being taken.