Method for preparing recombined apoptosis induction ligand relevant to human soluble tumor necrosis factor

Abstract

A process for preparing the wither inducing ligand (TRAIL) associated with recombinant human soluble tumor necrosis factor includes such steps as providing thue engizzeered bacterium rsTRAIL96-281-pET156-BL21 (DE3) (CGMCC No.0659), fermenting culture, washing, ultrasonic breaking, salting-out, chromatography and exchanging in buffering liquid system.

Description

technical field

[0001] The invention relates to the preparation of medicinal protein substances, in particular to a method for preparing target proteins by using genetically engineered bacteria, in particular to a preparation method for recombinant human soluble tumor necrosis factor-related apoptosis-inducing ligands. Background technique

[0002] Tumor is a common and frequently-occurring disease that seriously threatens human life, and its high fatality rate accounts for the second cause of all deaths. However, the feedback data after decades of clinical application shows that although chemotherapy is currently the preferred anti-tumor regimen, most anti-tumor chemotherapeutic drugs have two serious problems: strong toxic side effects on normal cells and drug resistance of tumor cells Therefore, cancer patients cannot receive effective treatment. While traditional chemotherapy drugs kill tumors, they "do not distinguish between the enemy and the enemy", have strong toxic...