Medium chain fatty acids applicable as anitmicrobial agents
A medium-chain fatty acid, a technology for inhibiting microorganisms, applied in the field of medium-chain fatty acids as microorganisms, and can solve the problems of not providing
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Embodiment 1
[0043] Example 1: Effect of MCFAs on growth and survival of fungi and yeast at pH 4 (mainly MCFA in acid form)
[0044] The following six fungal / yeast strains were evaluated: Penicillium chrysogenum MUCL 28658, Aspergillus niger MUCL 19001, Candida albicans MUCL 29800, Fusarium oxysporum MUCL 781, Cephalosporum chrysogenum MUCL9718 and Saccharomyces cerevisiae MUCL 31497. Each strain was grown at 25°C in a specific MCFA 50% / 50% wt (C with the following pH values and the following concentrations 8 -C 10 ) in P (potato) D (glucose) broth medium: 2400ppm (pH 4.0), 1200ppm (pH 4.0), 600ppm (pH 4.0), 300ppm (pH4.0), 0ppm (pH 4.0), 0ppm ( pH 7.0). After 7 days, all fermentation broths were evaluated for growth. To determine the type of inhibition (killing or quiescent models of action), plate counts were performed. Table 1 summarizes the growth results. No growth could be observed after plate counting on PD agar at 300 ppm of the specific MCFA and for all microbial strains. ...
Embodiment 2
[0048] Example 2: Effect of MCFA on growth and survival of fungi and yeast at pH 7 (mainly MCFA in salt form)
[0049] The same experimental conditions as described in Example 1 were used. However, in this experiment, all fermentation broth media were set at pH 7.0. The results are summarized in Table 2. Where the strain did not grow, the cells were also killed (as confirmed by plate counts on PD agar).
[0050] Table 2. Growth of fungi / yeast at pH 7.0 at various concentrations of MCFA ("+" growth, "-" no growth).
[0051] 0ppm,
pH 7
300ppm,
pH 7
600ppm,
pH 7
1200ppm,
pH 7
2400ppm,
pH 7
+
+
+
-
-
Saccharomyces cerevisiae
+
-
-
-
-
Aspergillus niger
+
+
-
-
-
Fusarium oxysporum
+
+
+
-
-
Cephalosporium chrysogenum
+
+
-
-
-
Penicillium chrysogenum
+
-
-
-
- ...
Embodiment 3
[0054] Example 3: In vivo evaluation of MCFA in early weaned piglets
[0055] The gastrointestinal tract of weaned piglets is very sensitive to E. coli K88 contamination. E. coli K88 contamination mainly resulted in diarrhea and reduced capacity (in response to daily growth and feed conversion). To describe the control of E. coli K88 contamination in piglets by means of MCFA, the following experiments were performed.
[0056] A mixture according to the present invention was prepared containing about 40 parts by weight of barley, 14 parts by weight of wheat, 10 parts by weight of corn product, 11 parts by weight of soybean product and 20 parts by weight of 8-10 Feed supplement composition for specific MCFAs of carbon atoms. Add 10 per gram of feed 8 A pathogenic bacteria (Escherichia coli K88).
[0057] A control feed was prepared containing the same components as the above mixture except that the control feed did not contain the specific MCFAs.
[0058] A group of 10 pigs...
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