Chitosan pellet/microsac and preparation thereof

A technology of chitosan microspheres and chitosan solution, which is applied in the fields of medical engineering and bioengineering, can solve the problem of low mechanical strength of chitosan microspheres/microcapsules, and achieves the solution of low mechanical strength and mild preparation process. , the effect of reducing the loss of activity

Inactive Publication Date: 2009-08-05
INST OF PROCESS ENG CHINESE ACAD OF SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome when prior art prepares chitosan microsphere / microcapsule, use emulsification dispersion cross-linking method or solvent volatilization method to introduce toxic cross-linking agent or organic solvent, use coacervation method or spray-drying method to prepare Chitosan microspheres / microcapsules have defects of low mechanical strength, so as to provide a kind of chemical cross-linking agent, organic solvent and other harmful substances, with controllable particle size, controllable surface state, controllable swelling property, mutual Non-adhesion, biodegradable chitosan microsphere / microcapsule, and preparation method thereof

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chitosan pellet/microsac and preparation thereof
  • Chitosan pellet/microsac and preparation thereof
  • Chitosan pellet/microsac and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Chitosan was dissolved in 0.2mol / L acetic acid solution to prepare a 2% (w / v) solution, 50mL of liquid paraffin was added to the reactor, 0.5mL of Span80 and 1mL of Tween80 were added, stirred for 10 minutes, and the rotating speed was 1500 rpm Minutes, after adding the 10mL chitosan solution prepared above, continue to stir and disperse for 30 minutes with the same rotating speed, then add 40mL of ammonium sulfate solution with a concentration of 50% (w / v), continue to stir for 1 hour and then stop. Centrifuge, pour out the upper liquid, separate the chitosan microspheres precipitated by ammonium sulfate in the lower layer, wash the precipitates with pure water, petroleum ether, and absolute ethanol in turn, and dry them in vacuum at room temperature to obtain the chitosan microspheres of the present invention. ball.

[0035] Its surface morphology is as figure 1 with figure 2 As shown in the scanning electron microscope picture, it can be seen that the chitosan mic...

Embodiment 2

[0037]Dissolve chitosan in 0.25mol / L acetic acid solution to prepare a 5% (w / v) solution, take 50mL peanut oil and add it to the reactor, add 0.5mL Span60 and 1mL Tween60, stir for 5 minutes, and the rotating speed is 2000 rpm After adding the above-mentioned chitosan solution of 10mL, continue to stir after 60 minutes with the same rotating speed, add the ammonium sulfate solution 100mL that concentration is 30% (w / v), stop after continuing to stir for 2 hours. Centrifuge, pour out the upper liquid, separate the chitosan microspheres precipitated by ammonium sulfate in the lower layer, wash the precipitates with pure water, petroleum ether, and absolute ethanol in turn, and dry them in vacuum at room temperature to obtain the chitosan microspheres of the present invention. ball.

[0038] Its surface morphology is as image 3 with Figure 4 As shown in the scanning electron micrograph, it can be seen that the chitosan microspheres have a porous surface, a particle size of 15...

Embodiment 3

[0040] Chitosan was dissolved in 0.25mol / L acetic acid solution to prepare a 4% (w / v) solution, 50mL corn oil was added to the reactor, 1mL Tween65 was added, stirred for 20 minutes, and the rotating speed was 500 rpm; After 2.5mL of the chitosan solution prepared above, continue to stir with the same rotating speed for 40 minutes, add concentration and be 50mL of ammonium sulfate solution of 65% (w / v), stop after continuing to stir for 3 hours. Centrifuge, pour out the upper liquid, separate the chitosan microspheres precipitated by ammonium sulfate in the lower layer, wash the precipitates with pure water, petroleum ether, and absolute ethanol in turn, and dry them in vacuum at room temperature to obtain the chitosan microspheres of the present invention. ball.

[0041] The cross-section of the microsphere is shown in Figure 5 As shown, it can be seen that its internal structure is dense and it is a solid ball.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention relates to a chitosan microsphere / microcapsule and a preparation method thereof. The chitosan microspheres / microcapsules are obtained by emulsification dispersion-ammonium sulfate precipitation method, including: stirring the oil phase and 0.1-4v% surfactant; adding 0.5-5% (w / v) chitosan solution (water phase), the volume ratio of chitosan solution to oil phase is 1:2~20, continue to stir; then add ammonium sulfate solution with a concentration of 1~65% (w / v), ammonium sulfate The volume ratio of the solution to the chitosan solution is 1:1-20, and the stirring is continued; the precipitate is separated by centrifugation, washed and dried to obtain the chitosan microsphere / microcapsule of the present invention. The chitosan microspheres / microcapsules obtained by the method have controllable particle size, controllable surface state, controllable swelling properties, non-adhesion, and biodegradability, and the preparation process of the method is mild without the use of toxic chemicals. Crosslinking agents and organic solvents.

Description

technical field [0001] The invention belongs to the fields of medical engineering and bioengineering, and specifically relates to a chitosan microsphere / microcapsule with controllable particle size, controllable surface state, controllable swelling property, non-adhesion, and biodegradability. and its preparation method. Background technique [0002] Chitosan [β-(1-4)-2-amino-2-deoxy-D-glucan] is the deacetylation product of chitin, which is abundant in nature, due to its good biocompatibility, biological Degradability and bioadhesion have been widely valued by researchers in the field of medicine and bioengineering materials. Chitosan microspheres / microcapsules have a large surface area to volume ratio, and can be used as a drug carrier to obtain slow-release and controlled-release effects to obtain ideal drug release properties; chitosan microspheres / microcapsules with a certain particle size have targeted It can concentrate the drug on the lesion, improve the therapeuti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/36A61K9/16
Inventor 李巧霞宋宝珍仰振球樊红雷
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap