Culture in vitro for silver magpie tree
A cultivation method and in vitro cultivation technology, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of low seed conversion rate, difficulty in fruiting, long interval period, etc., achieve expansion of reproduction coefficient, and broad application prospects , The effect of convenient material collection
Inactive Publication Date: 2009-11-04
CHINA THREE GORGES UNIV
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Problems solved by technology
Due to the dioecious male and hermaphroditic flowers of the silver magpie tree, pollination is more difficult, and it is not easy to bear fruit. At the same time, the species has a long interval between fruiting, low conversion rate of seeds to seedlings, and weak natural regeneration ability. In addition, natural trees have been cut down repeatedly. Its population is declining and endangered
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Embodiment 1
[0027] The in vitro cultivation of embodiment 1, silver magpie tree
[0028] MS basal medium: It can be prepared by referring to the literature (Murashige T, Skoog F. A revised medium for rapidgrouth and bioassays with tobacco tissue cultures. Physiol. Plant, 1962, 15: 473-497).
[0029] The in vitro culture method of silver magpie tree of the present invention may further comprise the steps:
[0030] 1) Obtaining sterile immature embryos
[0031] Rinse the fruit of the silver magpie tree with water, peel off the peel and seed coat, take out the seeds, and then disinfect the surface with 75% alcohol on the ultra-clean workbench for 30-90s, and then use 2 drops of soil temperature-40 per 100mL 0.1% HgCl 2 Disinfect the solution for 5-20 minutes, wash it with sterile water 4-6 times, each time for 1-3 minutes, put it in sterile water after cleaning, soak it at 4°C for 12-24 hours, and again with 0.1% HgCl 2 The solution is sterilized for 3-8 minutes, washed with sterile water...
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The invention discloses a method for in vitro cultivation of silver magpie tree. The culture method comprises the following steps: 1) induction of embryogenic callus: using sterile immature embryos as explants, placing them on an embryogenic callus induction medium to induce embryogenic callus; The embryogenic callus induction medium is added 2,4-D and activated carbon on the basis of MS basic medium; 2) differentiation of somatic embryos: embryogenic callus is placed on the differentiation medium to induce differentiation ; The differentiation medium added 6-benzyl adenine and α-naphthalene acetic acid on the basis of MS basic medium; 3) rooting and plant regeneration: the differentiated cotyledon embryos were placed on MS basic medium Take root and cultivate to obtain the regenerated plant of the silver magpie tree. The invention lays a good foundation for expanding the reproduction coefficient of the silver magpie tree, the germplasm preservation and genetic transformation of the species, and has broad application prospects.
Description
technical field [0001] The invention relates to an in vitro cultivation method of plants, in particular to an in vitro cultivation method of the rare plant silver magpie tree. Background technique [0002] Silver magpie tree (Tapiscia sinensis Oliv), also known as gall pepper tree, is a species of Stanhyleaceae, a relict plant of Quaternary glaciers, and has been listed as a third-level protected plant in my country. Silver magpie tree is a rare and endangered plant unique to my country. It is mainly distributed in Guizhou, Sichuan, Hunan, Hubei and Anhui provinces. Silver magpie tree has yellow flowers, aroma, yellow leaves in autumn, green in spring and summer, and red fruit. It is an excellent foliage and fruit tree species in landscaping; its leaves contain flavonoids, which are beneficial to expanding the plant resources and resources of flavonoids. The comprehensive utilization of silver magpie tree is also of great significance; in addition, the material of silver ma...
Claims
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IPC IPC(8): A01H4/00C12N5/04A01C1/00A01C1/08A01G17/00
Inventor 陈发菊梁宏伟王玉兵何正权姚伟王希群乐超银梁薇
Owner CHINA THREE GORGES UNIV