Rice chlorophyll synthase mutant gene and its application in gene engineering

Abstract

The invention discloses a nucleotide sequence of rice chlorophyll synthase mutation gene Osygl1 and gene engineering application in the gene engineering domain, which is characterized by the following: the cDNA sequence of Osygl1 is SEQ ID NO.1 and its coded amino acid sequence is SEQ ID NO.2; the gene OsYGL1 is a chlorophyll synthase gene in the rice, which participates the synthesis of chlorophyll a and chlorophyll b to display component expression; the mutation of the gene leads the leaf of rice to become yellow green, which can be genetic mark to identify the kind in the agricultural manufacturing and rice genetic breeding.

Description

technical field

[0001] The invention relates to a novel rice chlorophyll synthase mutant gene Osygl1 and its application in genetic engineering, belonging to the field of genetic engineering, and specifically relates to genes for rice chlorophyll synthesis and rice leaf color development. technical background

[0002] Plant leaf color is a comprehensive expression of various pigments in chloroplasts. In normal leaves, chlorophyll is dominant and usually appears green. Leaf color variation is a mutation trait with high mutation frequency and easy identification in higher plants. So far, leaf color mutants have been found in almost all higher plants such as rice, wheat, barley, corn, soybean, cotton, tobacco, corn, sunflower, tomato, cucumber, potato, and mulberry. The mutated gene directly or indirectly affects the synthesis and degradation of photosynthetic pigments, especially chlorophyll, resulting in changes in the content and proportion of various pigments, thereby caus...

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