Unlock instant, AI-driven research and patent intelligence for your innovation.

Recessive gene xa13 of rice bacterial blight resistance and its allelic dominant gene xa13

A dominant gene and recessive gene technology, applied in the field of plant biology, can solve the problems of crisp rice quality and reduced germination rate

Inactive Publication Date: 2009-12-23
HUAZHONG AGRI UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacterial blight causes crisp rice quality and reduced germination rate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Recessive gene xa13 of rice bacterial blight resistance and its allelic dominant gene xa13
  • Recessive gene xa13 of rice bacterial blight resistance and its allelic dominant gene xa13
  • Recessive gene xa13 of rice bacterial blight resistance and its allelic dominant gene xa13

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Construction of a physical map of the xa13 segment of the recessive gene resistant to bacterial blight

[0025] 1. Experimental materials and inoculation methods

[0026] The indica rice line "IRBB13" carrying the recessive gene xa13 and the near-isogenic line of "IRBB13" (recurrent susceptible parent), "IR24" carrying the dominant gene Xa13 (Chu et al., 2004, Genome-wide analysis ofdefense-responsive genes in bacterial blight resistance of rice mediated by the recessive Rgenexa13.Mol.Genet.Genomics 271:111-120) constructed three F 2 group. The first population consisted of 250 extremely resistant (lesion length less than 3 cm two weeks after inoculation with bacterial blight PXO99) individual plants, the second population consisted of 6000 random individual plants, and the third population consisted of 1972 individual plants Composition of random individual plants. Use the three F's 2 Population mapping of the recessive gene xa13.

[0027] The present i...

Embodiment 2

[0035] Example 2: Fine-mapping the recessive gene xa13

[0036] Molecular marker E6a in the second F 2 Recombination with the xa13 site was detected in 26 individual plants of the population ( figure 2 ). The 26 recombinant exchange individuals detected with the E6a marker were further analyzed with a series of molecular markers from the sequenced BAC clones. These markers include 6 shotgun clones (RP3, RP4, RP5, RP7, RP8 and RP10) derived from BAC clones 21H14 and 14L03, the SSR (simple sequence repeat) marker SR6 designed based on the 21H14 sequence and the CAPS marker ST9 designed based on the 14L03 sequence . The analysis found that the markers RP3, RP4, RP5, SR6 and RP7 were detected to recombine with the xa13 locus in 1 to 15 of the 26 individual plants related to the E6a marker, while the markers RP8, ST9 and RP10 were detected in the 26 individual plants. All of the individual plants were co-segregated with the xa13 locus ( figure 2 ).

[0037] The SSR marker S...

Embodiment 3

[0038] Example 3: Isolation and functional verification of the dominant gene Xa13

[0039] 1. Determination of Xa13 candidate genes

[0040] Using GenScan ( http: / / genes.mit.edu / GENSCAN.html ) and Fgenesh (http: / / www.softberry.com / ) gene prediction software for the allelic dominant gene Xa13 between molecular markers RP7 and ST9, including xa13, derived from rice variety IR64 14.8kb ( figure 2 ) sequence analysis, it was shown that there may be three genes in this segment. The first and third genes are incomplete and the second gene is complete. Therefore, it is speculated that the second gene is the dominant gene Xa13.

[0041] 2. Isolation and verification of Xa13 candidate genes

[0042] The present invention uses restriction endonuclease BamHI to digest the plasmid DNA of the BAC clone 14L03 of rice variety IR64, and isolates a candidate gene comprising the dominant gene Xa13, a DNA fragment about 14 kb in length ( image 3 ). The Agrobacterium-mediated genetic tra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of plant biotechnology. It specifically relates to the separation and cloning, functional verification and application of two DNA fragments comprising the rice bacterial blight-resistant recessive gene xa13 and the allelic dominant gene Xa13 of xa13. The disease resistance recessive gene xa13 is a functionally deficient mutant of the dominant gene Xa13. Mutation of the dominant gene Xa13 confers resistance to bacterial blight in rice. Using the transgenic technology based on the principle of RNA interference (RNA interference, RNAi), the recessive gene xa13 or the partial DNA fragment of the dominant gene Xa13 is connected with the exogenous regulatory sequence and transferred into rice to inhibit the function of the dominant gene Xa13 in rice. It can significantly enhance the resistance of rice to bacterial blight; in addition, by inhibiting the function of the recessive gene xa13 in rice through RNAi technology, the resistance of rice to bacterial blight can be further enhanced.

Description

technical field [0001] The invention relates to the field of plant biotechnology. It specifically relates to the isolation, cloning, functional verification and application of a rice bacterial blight-resistant recessive gene xa13. The disease resistance recessive gene xa13 is a functionally deficient mutant of its allelic (dominant) gene Xa13. The mutation of the dominant gene Xa13 confers resistance to some bacterial blight in rice. Background technique [0002] Plants are attacked by various pathogens during their growth. There are many types of plant pathogens, including viruses, bacteria, molds and nematodes. Pathogen invasion of plants leads to two results: (1) the pathogen successfully reproduces in the host plant, causing related diseases; (2) the host plant produces a disease-resistant response, killing the pathogen or preventing its growth. Using resistance gene resources to improve plant disease resistance is the fundamental way to prevent diseases and protect ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82
Inventor 王石平储昭晖袁猛葛小佳杨红
Owner HUAZHONG AGRI UNIV