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Extraction method of korean pine cone extractive

An extraction method and technology of pine cones of Korean pine, which are applied in the field of extraction of pine cone extracts, can solve the problems of low utilization rate and added value, and discard the pine cones of Korean pine, and achieve the improvement of utilization rate and added value, and the suppression of Tumor growth, the effect of simple and easy extraction methods

Inactive Publication Date: 2007-08-15
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because there is no record that the Korean pine cone can be used as medicine, the Korean pine cone is often discarded or only used as firewood and handicrafts, and there are problems of low utilization rate and added value

Method used

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Examples

Experimental program
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Effect test

specific Embodiment approach 1

[0006] Specific embodiment one: the present embodiment Korean pine cone extract is carried out according to the following steps: (1) the Korean pine cone is pulverized into the particle of 150~840 μm, then with concentration is the ethanol reflux extraction of 95% 3~4 times, Each reflux extraction is 2 ± 0.1h, and the amount of ethanol used for each reflux extraction is 5 to 10 times the quality of the Korean pine cone; (2) Combine the extracts and concentrate under reduced pressure to obtain the extract; (3) Extract the extract with a volume of 3 ~5 times of absolute ethanol to dissolve the extract, then add the extract alcohol solution in batches to silica gel with 2.5 to 3.5 times the mass of the extract, stir evenly, and dry at 60±2°C; (4) Column packing (5) eluting, promptly obtains the Korean pine cone extract.

specific Embodiment approach 2

[0007] Specific embodiment two: the difference between this embodiment and specific embodiment one is: in the step (1), the consumption of each reflux extraction ethanol is 6~8 times of the quality of the Korean pine pine tower. Other steps and selected parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0008] Specific embodiment three: the difference between this embodiment and specific embodiment one is: in the step (1), the consumption of each reflux extraction ethanol is 7 times of the quality of the Korean pine pine tower. Other steps and selected parameters are the same as those in Embodiment 1.

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Abstract

The invention relates to a method for extracting effective components from pinecone of Pinus koraiensis, which solves the problems of low utilization rate and added value of Pinus koraiensis. The method comprises (1) extracting with ethanol under reflux; (2) concentrating under reduced pressure to obtain an extract; (3) dissolving the extract in anhydrous alcohol, mixing with silica gel; (4) filling the chromatographic column; and (5) eluting to obtain the extract of Pinus koraiensis. The invention adapts organic solvent as the extractant with high extraction rate of 16.8-17.5%. The method has the advantages of simple extraction, low difficulty, reduced loss of effective components as the solvent and gel has no affect to the extract, and easy operation. The extract of Pinus koraiensis pinecone has anti-inflammatory and antitumor effects, and is used to produce pharmaceuticals with high added value and utilization rate.

Description

technical field [0001] The invention relates to a method for extracting pine cone extract. Background technique [0002] Baisongta (Baisongsongta), as a traditional Chinese medicine, has the effects of antitussive, expectorant, anti-inflammatory, and asthma; it can treat chronic bronchitis, cough, shortness of breath, and spit white foamy sputum. At present, the hot water extraction method is used to extract the white pine cone. Its water extract is mainly acidic polysaccharides, and its activity increases with the enhancement of acidity. It can prolong the survival time of mice infected with ascites tumor cells and reduce the mouse mammary tumor virus ( MMTV) content and the effect of effectively preventing the increase of the mouse mammary tumor virus (MMTV) content in milk; and in the white pine cone alkaline aqueous solution, high molecular weight active components with anti-AIDS virus (HIV) activity can also be extracted. But the pH value of solution in the white pine ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/15A61K9/16A61P29/00A61P35/00A61K131/00
Inventor 杨鑫张华张英春王静
Owner HARBIN INST OF TECH
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