Extract-agitating self-suction type biological reactor

A technology of bioreactor and reactor, which is applied in the direction of bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, biochemical instrument, etc. To avoid problems such as poor quality and effect, to increase the effect of gas-liquid mixing, easy to operate, and simple in structure

Inactive Publication Date: 2007-08-29
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the research on petroleum biological desulfurization is in the basic research stage. There are only a few researches on reactors. The ones that have been reported mainly include stirred tank reactors, airlift reactors, free cell milk phase contact reactors, and immobilized reactors. Cell fluidized bed reactor, etc. (referring to literature 2: McFarland BL, et al.Crit.Rev.Microbiol., 1998, 24:99~147; Literature 3: MonticelloD J.USP 5472875.1995; Literature 4: Kaufman E N, et al .Appl.Biochem.Biotech., 1998, 73:127~144), these reactors have their own advantages and disadvantages: wherein the stirred tank reactor is used most; the emulsion contact reactor can produce 5 μm water / oil / bacteria droplets, It achieves full mixing of the oil phase and the water phase, but has a low desulfurization rate; while the fluidized bed reactor immobilizes the biocatalyst on a suitable carrier, which is easy for continuous operation and product separation, but the immobilization of biocatalyst and other technologies Further research is needed; these reactors are not only complex in structure and occupy a large area, but also have high energy consumption, low efficiency, and poor mass transfer effect
So far, no reports have been found on bioreactors that combine stirring, self-priming and extraction and spraying

Method used

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  • Extract-agitating self-suction type biological reactor
  • Extract-agitating self-suction type biological reactor
  • Extract-agitating self-suction type biological reactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Referring to Figures 1 to 3, a stirred liquid self-priming bioreactor that can be used for cell fermentation reactions is produced according to the connection relationship of the aforementioned reactors.

[0034] The stirred liquid self-priming bioreactor provided in this example has an inner diameter of 74 mm, a height of 135 mm, a volume of 500 mL, and an effective volume of 200 mL. The upper half of the central shaft is 110mm long and has an inner diameter of 6mm; the lower half is 70mm long and has an inner diameter of 25mm. The outer cavity sleeve (gas phase sleeve) is 70mm high, and two Ф5mm small holes are opened at the upper end of the outer cavity sleeve at 5mm, and two gas phase centrifugal forces with a length of 10mm and an inner diameter of Ф2mm are connected at the lower end of the outer cavity sleeve at 5mm. Tube channel. The inner cavity sleeve (liquid phase sleeve) is attached to the outside of the outer cavity sleeve, the lower end is open, and the he...

Embodiment 2

[0036] Made according to Example 1, and in the reactor that has been made in Example 1, an electric heating tube is installed, and the heating tube is electrically connected with an external temperature controller, or an external heat exchange type ventilation tube is placed in the lower part of the reactor shell. The jacket of hot and cold water is electrically connected with the external temperature controller.

Embodiment 3

[0038] The fermentative culture of the biocatalytic desulfurization strains was carried out in the stirred liquid self-priming bioreactor made in Example 1 or Example 2. The steps are as follows:

[0039] (1) Seed culture of biological desulfurization bacteria

[0040] Take 0.5 mL of Pseudomonas delafildii R-8 strain (Pseudomonas delafildii R-8, CGMCC NO.0570) preserved in glycerol, add it to the medium with a volume of 20-50 mL, and put it under the condition of 30°C and 170r / min Cultivate for 1 to 2 days;

[0041] The composition of the medium is 2.44 g KH per liter of water 2 PO 4 , 14.03 g Na 2 HPO 4 12H 2 O, 0.4g MgCl 2 ·6H 2 O, 0.001g CaCl 2 2H 2 O, 0.001g FeCl 3 ·6H 2 O, 0.004gMnCl 2 4H 2 O, 10 g glycerol and 2.00 g NH 4 Cl. The sulfur source is dibenzothiophene with a concentration of 0.1 mmol / L.

[0042] (2) Stirring liquid self-priming bioreactor experiment

[0043] Add 200 mL of the above-mentioned culture medium into the reactor, and sterilize wit...

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PUM

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Abstract

The invention relates to gas-liquid, gas-liquid-solid or gas-liquid-liquid multiphase biology chemical operation reactor. It contains a reaction carcase, an upper cover and aseptic filtering film, and sampling connection. The upper end of center rotating shaft connects to the shaft of electric mixer, and the down end has self-sucking whisking liquid lifter. An air intake is on the upper end of inner cavity sleeve, and a gas phase centrifugal force pipe type passage is on the down part of the inner cavity sleeve. Outer cavity sleeve is installed on outside wall of inner cavity sleeve, and liquid phase centrifugal force pipe type passage connects to the upper part. The invention realizes whisking, mixing and air feeding process to improve oxygen dissolving efficiency.

Description

technical field [0001] The invention relates to a gas-liquid two-phase, gas-liquid-solid or gas-liquid-liquid multi-phase biochemical operation reactor, in particular to a stirring liquid extraction self-priming bioreactor suitable for aerobic liquid biological fermentation reaction . Background technique [0002] A bioreactor is a device that provides a good reaction environment for biocatalysts (microbial cells or enzymes) to complete biocatalytic reactions, often called fermenters. The characteristics of an excellent bioreactor are simple equipment, not easy to infect bacteria, low energy consumption, good liquid mixing performance, high mass transfer and heat transfer rate, and high production capacity per unit time and unit volume. , Operation control and maintenance are convenient, easy to enlarge and so on. The fermentation reactor for cultivating microorganisms must have various conditions suitable for the growth of microorganisms and the formation of products (suc...

Claims

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Application Information

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IPC IPC(8): C12M1/02
CPCC12M27/02C12M29/06
Inventor 李玉光刘会洲安震涛李望良熊小超李信邢建民
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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