Preparation method of oyster active peptides

A technology of active peptides and oysters, applied in the fields of peptide sources, animal/human peptides, anti-tumor drugs, etc.

Inactive Publication Date: 2007-09-19
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, developed countries such as Europe, Japan, and the United States have increasingly conducted research on biologically active substances i

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0009] Example 1:

[0010] Shuck the oysters, take the oyster meat, wash it with 4°C water, homogenize it, add distilled water at a weight ratio of 1:1 and extract at low temperature (4°C) for 2 hours, flocculate, centrifuge to remove slag, take the supernatant, and put the After the supernatant was lyophilized, it was separated on a Sephacryl s-100 HR column (0.03mol / lNH 4 HCO 3 Buffer elution), collect each peak separately, take the peak with biological activity and then use anion sepharose DEAE-Sepharose-FF ion exchange column chromatography (pH8.5 Tris-HCl buffer, 0~0.3mol / lNaCl Gradient elution), collect active peaks, freeze-dry, and desalt with Sephadex G15 gel column to obtain pure oyster natural active polypeptide.

Example Embodiment

[0011] Example 2:

[0012] Shell the oysters, wash, homogenize, add distilled water at a weight ratio of 1:1, add trypsin at a weight ratio of 2% of the substrate, enzymolysis at 45°C for 4 hours, kill the enzyme, centrifuge to remove the residue, and freeze-dry to obtain the enzyme The crude extract was dissolved, and the crude extract was subjected to gel column chromatography with Sephadex G25 to collect each peak, and then separated and purified by anion sepharose DEAE-Sepharose-FF ion exchange column chromatography (PH8. 5 Tris-HCl buffer, 0-0.3 mol / l NaCl gradient elution), collect active peaks, freeze-dry, and desalt with Sephadex G15 gel column to obtain pure oyster natural active polypeptide. The active peptide is a naturally extracted substance and belongs to an oligopeptide with a small molecular weight, and the extraction process effectively protects its biological activity.

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Abstract

The invention relates to a method for hydrolyzing oyster protein by enzyme method and extracting oyster active peptide by column chromatography including: rinding the oyster, washing, homogenate, adding distilled water to extract the oyster liquid at a low temperature; flocculating, centrifugal deslagging, taking the supernatant fluid, freeze-dry the supernatant fluid, separateing with the Sephacryl s-100 HR column or enzymolysis, inactivating, centrifugal deslagging, freeze-dry the oyster liquid to get coarse extract, gel column chromatography of the coarse extract; collecting the respective peak, purifying with ion-exchange column chromatography, collecting the respective peak having activity, desalting with the gel column after freeze out and getting the oyster natural purified activity peptide. The oyster natural activity peptide has a high purity and has an anti-tumor activity. The active peptide is natural-extracted and is protected in the extraction process and may be a medicine to use.

Description

Technical field: [0001] The invention relates to a preparation method of oyster active peptide, specifically a method for hydrolyzing oyster protein by enzymatic method and separating and purifying oyster active peptide by ultrafiltration and column chromatography, belonging to the technical field of marine natural products and medicine. Background technique: [0002] Oyster (Oyster) is a kind of marine shellfish. Oysters belong to Molluscs, Phallobranches, Heterocylinda, Oyster Superfamily, and Oysteridae in classification. It is a bivalve seafood that is used for both food and medicine. It is cultivated in the north and south coasts of my country, especially in Guangdong Province, which has developed rapidly in recent years. The oyster meat is milky white, tender, and rich in nutrients. In addition to rich protein, vitamins, sugar and other nutrients, it also contains more than ten essential nutrients. Amino acids, mineral nutrients, etc. Current studies have also proved ...

Claims

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Application Information

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IPC IPC(8): C07K1/14C12P21/00C07K14/435A61P35/00
Inventor 韩玉谦冯晓梅赵志强蒋新管华诗
Owner OCEAN UNIV OF CHINA
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