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Preparation method for extracting purified general saponin from notoginseng of Chinese traditional medicine

A technology for total saponins and Panax notoginseng, applied in the field of medicine, can solve the problems of low content of controllable components, influence on the safety and effectiveness of Panax notoginseng saponins, and disadvantages, and achieves low cost, easy industrialized production, and simple operation. Effect

Inactive Publication Date: 2007-10-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In patent 2 (CN93105774.4) and patent 3 (CN02131193.5), the content of saponins in the product measured by high performance liquid chromatography is 88% and 60%, respectively, and the content of controllable components is not high, which will inevitably affect the total saponins of Panax notoginseng in the product. The safety and effectiveness of clinical drugs are also not conducive to breaking through the technical barriers to enter the international market

Method used

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  • Preparation method for extracting purified general saponin from notoginseng of Chinese traditional medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 500g of the coarse particle of Panax notoginseng (particle diameter about 1cm), dry at 50°C for 6 hours, add 8 times the amount of 30% ethanol and heat it under reflux to extract twice, 1 hour each time, filter the extract, combine, and concentrate under reduced pressure To 0.5g / mL (crude drug amount), go to D 101 Macroporous resin. Rinse with 4 times the column volume of deionized water, discard, continue to rinse with 4BV 30% ethanol solution, discard, and finally elute with 5BV 70% ethanol solution, collect the eluate, concentrate under reduced pressure to no alcohol, freeze-dry , that is, Panax notoginseng total saponins. As determined by HPLC, the notoginseng saponin R in the refined 1 2%, Ginsenoside Rg 1 15%, Ginsenoside Rb 1 9%, ginsenoside Rd 3%, ginsenoside Re 1%, the total saponin content reaches 30%.

Embodiment 2

[0022] Take 500 g of the coarse particle (particle diameter of about 1 cm) of the medicinal material of notoginseng, dry it at 50°C for 6 hours, add 15 times the amount of 40% ethanol and heat it under reflux for extraction once in a slightly boiling state, for 2 hours, filter the extract, combine it, and concentrate it under reduced pressure to 0.5 g / mL (crude drug amount), above D 101 Macroporous resin. Rinse with 5 times the column volume of deionized water, discard, then rinse with 4BV of 1% ammonia water, discard, then rinse with deionized water until the pH of the effluent = 7, and finally elute with 6BV of 30% ethanol solution, collect the washed Remove liquid, concentrate under reduced pressure to no alcohol, and freeze-dry to obtain total saponins of Panax notoginseng. As determined by HPLC, the notoginseng saponin R in the refined1 4%, Ginsenoside Rg 1 30%, Ginsenoside Rb 1 15%, ginsenoside Rd 5%, ginsenoside Re 3%, the total saponin content reaches 57%.

Embodiment 3

[0024] Take 500g of the coarse particle of Panax notoginseng medicinal material (particle diameter about 1cm), dry at 50°C for 6 hours, add 18 times the amount of 50% ethanol and heat and reflux in a slightly boiling state to extract once for 1 hour, filter the extract, combine, and concentrate under reduced pressure to 0.5 g / mL (crude drug amount), above D 101 Macroporous resin. Rinse with 4 times the column volume of deionized water, discard, then rinse with 4BV of 0.1% ammonia water, discard, then rinse with deionized water until the pH of the effluent = 7, and finally elute with 5BV of 60% ethanol solution, collect the washed Remove liquid, concentrate under reduced pressure to no alcohol, and freeze-dry to obtain total saponins of Panax notoginseng. As determined by HPLC, the notoginseng saponin R in the refined 1 4%, Ginsenoside Rg 1 26%, Ginsenoside Rb 1 16%, ginsenoside Rd 5%, ginsenoside Re 4%, the total saponin content reaches 55%.

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Abstract

A process for extracting arasaponin from notoginseng and purifying it includes such steps as pulverizing the notoginseng, removing impurities, baking, percolating by the aqueous solution of alcohol, extracting by hot reflux, vacuum concentrating, adsorbing by macroreticular resin, eluting sequentially with water, ammonia water and alcohol solution, collecting the eluting liquid, concentrating, and drying. It can be made into tablet, capsule, or injection.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to obtaining total saponins of Panax notoginseng through extraction and refining of the traditional Chinese medicine Panax notoginseng. Background technique [0002] At present, Chinese herbal medicine has a broad market all over the world. With the increase of people's awareness of health requirements and the aging of the population, sub-health status, people are more eager to return to nature, using natural plants to treat and prevent some chemical synthesis. Diseases that are refractory to drugs. It has become the goal pursued by pharmaceutical companies all over the world to seek medicines with low side effects and high quality and low price from natural medicines. The European Community has carried out unified legislation on herbal medicines, and the status of herbal medicines in countries such as Canada and Australia has been legalized. The US government has also drafted a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258C07H1/08A61P9/10A61K125/00
Inventor 贺庆刘雳程翼宇瞿海滨水文波窦静霍杨葛志伟
Owner ZHEJIANG UNIV
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