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Production technique of green trichoderma by using mushroom slag fermentation

A fermentation process and technology of mushroom dregs, applied in the field of preparation of biological bacterial agents, can solve the problems of long survival time of antagonistic bacteria, achieve the effects of improving survival and reproduction ability, reducing residues, and supplementing soil nutrients

Inactive Publication Date: 2007-10-31
텐진인스티튜트오브플랜트프로텍션
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AI Technical Summary

Problems solved by technology

After 4 weeks of culture in sterile soil, the population density of antagonistic bacteria is 50-100 times higher than that of single application of antagonistic bacteria, and the survival time of antagonistic bacteria is longer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] Raw material processing: Select mushroom dregs without mildew and dry them in the sun and crush them into 80-mesh powder for later use.

[0013] Liquid culture: 100ml of liquid medium in a 500ml Erlenmeyer flask, 121°C, 20 minutes. After cooling, inoculate 10 ml of Trichoderma spore suspension, 26 ° C, 180 r shaking culture, 3 ~ 5 days, to obtain liquid strains. Add 10% (V / V) inoculation amount into the seed fermenter for fermentation and culture, the fermentation temperature is 25-28°C, ventilate and stir, ferment for 72 hours, and the spore content is 10% when the bacterial liquid is exported. 7 ~10 8 cfu / ml. Liquid medium 1L: 5-10 grams of yeast powder, 20-30 grams of starch, 10 grams of soybean milk, 2-5 grams of CaCO 3 , 0.3-0.5 g KH 2 PO 4 , 0.1-0.2 g MgSO 4 , PH is 5.5-6.5.

[0014] Solid fermentation: Prepare the medium according to a certain ratio, sterilize at 121°C for 30 minutes, put the solid medium into the fermentation tank after cooling, add 3-5 t...

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Abstract

The invention discloses a preparing method of bacterial agent in agricultural applied microbiology technical domain, which comprises the following steps: choosing mushroom slag as raw material; adopting liquid and solid two-phase method to produce bacterial agent. This invention possesses cheap cost and simple craft, which can be used to prevent and cure soil-borne disease widely.

Description

[0001] Technical field The present invention is a preparation method of biological bacterial agent, which belongs to the technical field of agricultural application microorganisms. Background technique [0002] The patent of the invention is to solve the frequent application of chemical pesticides at present, and the pesticide residues are continuously accumulated in vegetables and soil, and the application of Trichoderma viride has obvious preventive effect on various fungal soil-borne diseases. Use organic additives as carriers to multiply Trichoderma strains in large quantities to further consolidate the synergistic effect of the two, which not only supplements soil nutrients and enhances the antibacterial effect, but also improves the survival and reproduction of antagonistic bacteria in the soil and reduces the residue of pesticides in the soil. . [0003] There have been a large number of reports on the biological control of Trichoderma against soil-borne pathogens at ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/885
Inventor 郝永娟王正祥王万立刘春艳王勇杨秀荣
Owner 텐진인스티튜트오브플랜트프로텍션
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