Hainan sporic scorpion antibiotic and preparation method and application

A technology of antimicrobial peptide and scorpion venom, which is applied in the separation and identification of scorpion venom antibacterial peptide and scorpion antimicrobial peptide gene, and the field of preparation of the antimicrobial peptide gene, which can solve problems that do not involve the antibacterial function of scorpion toxin
CN101063103AInactive Publication Date: 2007-10-31WUHAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN UNIV
Publication Date
2007-10-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparing method of HaiNan speckle equal scorpion antibiotic peptide gene and appliance, which comprises the following steps: restructuring bacillus coli Escherichia coli DH5a / ImAMP1, CCTCC NO: M207034; constructing HaiNan speckle equal scorpion ioterium cell cDNA library; designing primer; choosing PCR method; sieving positive colony of scorpion antibiotic peptide gene from ioterium cDNA library; sequence-analyzing coding trait of antibiotic peptide gene; assuring amino acid sequence of the antibiotic peptide gene; adopting chemosynthesis antibiotic peptide; possessing inhibition of diverse density for Gram's bacterium. This peptide can be used to medicine to prevent and cure Gram's bacterium.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and the invention relates to a scorpion venom antibacterial peptide such as Hainan spot, a preparation method of the antibacterial peptide gene, application of the gene, molecular design and antibacterial function identification of the highly active antibacterial peptide. Specifically, the present invention relates to the isolation and identification of a scorpion antimicrobial peptide gene such as Hainan spot; it relates to the antibacterial spectrum identification of a scorpion antibacterial peptide: the scorpion venom antibacterial peptide produced by chemical synthesis means is effective against Gram-positive bacteria Has broad-spectrum resistance, but has no effect on Gram-negative bacteria; involves scorpion antimicrobial peptides that are effective against clinically isolated drug-resistant hemolytic staphylococci; also involves a highly active molecular design and antibacterial function identifi...

Claims

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