Method for preparing taxanes by epimerization biological transformation 7-epimer taxanes

Abstract

The present invention relates to a method of forming taxane diterpene by difference transformation 7- taxane using microorganisms. The present invention comprised the following steps: 1. Completely dissolve the 7- taxane compound which has a content of over 1% into the organic solvent that can be mixed with water at any ratio; 2. Add the solution of step 1 into the microbes biological culture, or abstraction enzyme from themicro-organism. An alternative is that after the extractenzyme immobilization, the solution of step 1 is added and the conversion time is 3-100 hours; 3. Extract taxane diterpene using conventional methods from the convert product of step 2. The technical problem solved in this invention is that the no valueble 7- taxane is converted into a valuble enantiomer, so as to save the resources of Yew.

Description

technical field

[0001] The invention relates to a method for converting 7-epimine taxane into taxane by means of microorganism epigenesis. technical background

[0002] Paclitaxel is an expensive anticancer drug extracted from the yew plant, and the production raw materials are seriously insufficient, resulting in high prices of paclitaxel. By biotransformation, the 7-epimized taxanes, which currently have no medicinal value and are present in a large amount in the branches and leaves of Taxus chinensis, are converted into paclitaxel and the precursor of paclitaxel that can be used for semi-synthesis, which can increase the yield of paclitaxel. Has great economic value. There are many kinds of epitaxane compounds, among which 7-epitaxol, 10-deacetyl-7-epitaxol, 7-epiacephalomannine, 10-deacetyl-7- Different to tricuspid Shanning, 10-deacetyl-7-different to baccatin III and so on. Their common feature in structure is that the 7-position hydroxyl group on the taxane mother ...