Method for crystallizing protein by using ion liquid

An ionic liquid and protein technology, applied in the field of protein crystallization, to achieve the effects of high diffraction intensity, good repeatability and improved operability
CN101092445AInactive Publication Date: 2007-12-26HARBIN ENG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HARBIN ENG UNIV
Publication Date
2007-12-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

This invention provides a method for crystallizing proteins from protein solution with ionic liquid-water mixed solution. The ionic liquid-water mixed solution is composed of ionic liquid 1-85wt.%, and buffer solution 1-25 wt.%, and the pH value is 4.0-11.0. The method has a wide process conditions, and can obtain protein single crystals with different morphologies under different conditions. The obtained protein single crystals have such advantages as high diffractive intensity, high repeatability and high operability.
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Description

(1) Technical field

[0001] The present invention relates to a new method of protein crystallization, in particular to a new method of using ionic liquid for protein crystallization. (2) Background technology

[0002] In recent years, due to the wide application of biologically active substances in medicine, pharmacy, chemistry, agriculture and industry, it is particularly important to reveal the relationship between the structure and function of biological macromolecules by X-ray crystallography. Biomacromolecules in crystal form are relatively stable, and X-ray diffraction is the most reliable method to determine the three-dimensional structure of crystal molecules. A detailed understanding of the structure of biological macromolecules is of great significance for the design of new drugs, protein engineering, and changes in molecular structure or conformation. But generally speaking, the crystal cultivation of biomacromolecules is still exploratory and empirical, and to so...

Claims

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