Ceanothus polysaccharide sulfate and its preparing process and application
A technology of rhamnosan and sulfate, applied in blood diseases, extracellular fluid diseases, drug combinations, etc., can solve the problems that rhamnosulphate has not been reported, and achieve good market application prospects
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[0010] Weigh the dried seaweed body and add 10-60 times the weight of the seaweed to soak the homogenate, stir and extract at room temperature for 0.1-2 hours, centrifuge to discard the clear liquid, take the sediment containing the seaweed body, and add the seaweed weight 10~ 60 times the water, stir and extract for 1-10 hours in a 60-100℃ water bath, centrifuge to remove impurities, take the clear liquid, concentrate the resulting clear liquid under reduced pressure, and then perform dialysis or ultrafiltration to remove the salt. After the polysaccharide solution is concentrated under reduced pressure, it is separated by ion exchange chromatography column Q-Sepharose Fast Flow, and then eluted with distilled water and 0.01~5.0mol / L sodium chloride aqueous solution. The polysaccharide solution is collected, concentrated under reduced pressure, and then passed through The gel chromatography column Sephacryal S-400 HR is further separated, eluted with distilled water, the polysacc...
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