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Tissue culturing method for chia

A technology of sage and tissue culture, applied in the field of bioengineering, can solve the problems that the tissue culture of sage has not been reported, and achieves the effects of shortening the culture time, large quantity and easy sampling.

Inactive Publication Date: 2008-02-13
HUNAN AGRICULTURAL UNIV
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AI Technical Summary

Problems solved by technology

At present, there are a few reports on the tissue culture of Salvia farinaeea. Wang Zongxia et al. obtained the regenerated plant of Salvia farinaeea. The regenerated plants of medicinal sage (Salavia of J icinalis I.) were cultivated (Journal of Xinjiang Agricultural University 2006, 29(4)), but the tissue culture of Chia sage has never been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Materials: chia sage (salvia hispanica L) seeds as explants,

[0021] 1. Disinfection of explants and waste liquid treatment: choose chia seeds as explants, disinfect them with conventional methods, treat them with 70% alcohol for 30 seconds on a sterile operating table, and then use 0.1% mercuric chloride Disinfect for 10-12 minutes, and finally rinse with sterile water for 5-6 times, and suck out the disinfected alcohol, mercury chloride water and sterile water with a 1000ul pipette;

[0022] 2. Inoculation method and induction of callus and adventitious buds: Sterilized chia seeds were sucked with a 1000ul pipette gun and inoculated in 1 / 2MS+6-BA10.0mg / L+NAA1.0mg / L , on the callus induction medium with a pH value of 5.8, inoculate 10-15 seeds per bottle in the inoculation bottle, culture in the culture room with low light, and keep the temperature at 24±2°C; the seeds start to germinate in about 3 days, and continue to culture for 12 days , began to form callus, and...

Embodiment 2

[0027] Chia sage (salvia hispanica L) seeds are explants, and the basic operation method is consistent with Example 1, but its callus, adventitious bud induction and proliferation medium are 1 / 2MS+6-BA5.0mg / L +NAA0.5mg / L medium, the seeds began to germinate in about 3 days, and after 15 days of continuous culture, callus tissue began to form, and at the same time clustered buds were differentiated, and a complete plant was formed in about 40 days, and a large number of differentiated tissue culture seedlings were obtained after 50 days. The multiplication factor is 4-5.

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Abstract

A method for tissue culture of callus and cluster buds of Chia sage. The invention adopts the regeneration principle of chia leaves, and the chia seeds are sterilized and inoculated in 1 / 2MS+6-BA5.0~10.0mg / L+NAA0.5~1.0mg / L callus On the induction medium, the seeds began to germinate after 3 days, and the culture continued for 14 to 16 days. Callus tissue began to form on the edge of the leaves, and clusters of adventitious buds were produced from the middle of the two cotyledons; NAA0.2~0.5mg / L on the rooting medium, grow roots to obtain regenerated shoots. The method of the invention simplifies the operation process, greatly shortens the cultivation time, saves the production cost, can be produced in batches, and has high application value. It is suitable for tissue culture of various sage species, and can quickly obtain tissue culture seedlings of sage, and can also provide a short cycle, high reproduction rate and low cost for large-scale planting of sage in production. Seedling method.

Description

technical field [0001] The invention belongs to the field of bioengineering and relates to plant tissue culture technology, in particular to a tissue culture method of chia sage. Background technique [0002] Chia sage (salvia hispanica L) is a summer annual herb of the Lamiaceae genus Salvia, native to Mexico and Central America. Commercial cultivation is mainly distributed in Mexico, Argentina, Belgium, the United States, Colombia, Bolivia, Peru and other regions. Chia has a long history of cultivation in the American continent. People have discovered and understood this plant to a certain extent as early as in pre-Columbian Central America. Chia seeds with high nutritional value have played an important role in the survival and development of human beings. Centuries ago in many parts of the Americas, chia seeds became the primary food source due to their high energy and high hunger tolerance, overshadowing traditional grains. The oil extracted from the seeds also has m...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 易自力黄丽芳蒋建雄陈智勇刘亮贾晓颜
Owner HUNAN AGRICULTURAL UNIV
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