Tissue culturing method for chia

A technology of sage and tissue culture, applied in the field of bioengineering, can solve the problems that the tissue culture of sage has not been reported, and achieves the effects of shortening the culture time, large quantity and easy sampling.

Inactive Publication Date: 2008-02-13
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are a few reports on the tissue culture of Salvia farinaeea. Wang Zongxia et al. obtained the regenerated plant of Salvia farinaeea. The regenerated plants of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example 1

[0020] Source: Salvia hispanica (salvia hispanica L) seeds as explants,

[0021] 1. Disinfection of explants and treatment of waste liquid: use Chia sage seeds as explants, disinfect by conventional methods, treat with 70% alcohol on a sterile operating table for 30 seconds, and then use 0.1% mercury Sterilize for 10-12 minutes, and finally rinse with sterile water for 5-6 times. Use a 1000ul pipette to suck out the sterilized alcohol, mercury and sterile water;

[0022] 2. Inoculation method and induction of callus and adventitious buds: The seeds of Salvia officinalis after disinfection are sucked with a 1000ul pipette and inoculated in 1 / 2MS+6-BA10.0mg / L+NAA1.0mg / L , PH 5.8 callus induction medium, inoculate 10-15 seeds per bottle in the inoculation bottle, cultivate in low light in the culture room, keep the temperature at 24±2℃; the seeds begin to germinate in about 3 days, and continue to cultivate for 12 days , Start to form callus, and at the same time d...

Example Embodiment

[0026] Example 2

[0027] The seeds of Salvia hispanica L are explants. The basic operation method is the same as that in Example 1, but the medium for callus, adventitious bud induction and proliferation is 1 / 2MS+6-BA5.0mg / L +NAA0.5mg / L medium, the seeds begin to germinate in about 3 days, and after 15 days of continuous cultivation, callus will begin to form, and cluster buds will be differentiated at the same time. Complete plants will be formed in about 40 days, and a large number of differentiated tissue culture seedlings will be obtained after 50 days. The multiplication factor is 4~5.

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Abstract

A method for tissue culture of callus and cluster buds of Chia sage. The invention adopts the regeneration principle of chia leaves, and the chia seeds are sterilized and inoculated in 1/2MS+6-BA5.0~10.0mg/L+NAA0.5~1.0mg/L callus On the induction medium, the seeds began to germinate after 3 days, and the culture continued for 14 to 16 days. Callus tissue began to form on the edge of the leaves, and clusters of adventitious buds were produced from the middle of the two cotyledons; NAA0.2~0.5mg/L on the rooting medium, grow roots to obtain regenerated shoots. The method of the invention simplifies the operation process, greatly shortens the cultivation time, saves the production cost, can be produced in batches, and has high application value. It is suitable for tissue culture of various sage species, and can quickly obtain tissue culture seedlings of sage, and can also provide a short cycle, high reproduction rate and low cost for large-scale planting of sage in production. Seedling method.

Description

technical field [0001] The invention belongs to the field of bioengineering and relates to plant tissue culture technology, in particular to a tissue culture method of chia sage. Background technique [0002] Chia sage (salvia hispanica L) is a summer annual herb of the Lamiaceae genus Salvia, native to Mexico and Central America. Commercial cultivation is mainly distributed in Mexico, Argentina, Belgium, the United States, Colombia, Bolivia, Peru and other regions. Chia has a long history of cultivation in the American continent. People have discovered and understood this plant to a certain extent as early as in pre-Columbian Central America. Chia seeds with high nutritional value have played an important role in the survival and development of human beings. Centuries ago in many parts of the Americas, chia seeds became the primary food source due to their high energy and high hunger tolerance, overshadowing traditional grains. The oil extracted from the seeds also has m...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 易自力黄丽芳蒋建雄陈智勇刘亮贾晓颜
Owner HUNAN AGRICULTURAL UNIV
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