Nano montmorillonite for restraining bacterium and method of preparing the same
A kind of montmorillonite and nanotechnology, which is applied in the direction of antibacterial drugs, inorganic non-active ingredients, active ingredients of tetracycline, etc., can solve the problems of unforeseeable technical effects and inferring antibacterial drugs, etc.
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Embodiment 1
[0038] 1. Preparation of Na-montmorillonite: the raw material montmorillonite (mainly containing Na + , Ca 2+ , Mg 2+ Ion) 20g was stirred in 500mL NaCl saturated deionized aqueous solution to form a stable suspension; the suspension was stirred at 35°C for 5 hours. Filter with suction, and then wash the filtrate 4 times with deionized water. The precipitate was vacuum-dried at 70°C to constant weight, and ball-milled to a particle size of 20-30 μm to obtain Na-montmorillonite (the XRD pattern is shown in figure 1 , whose infrared spectrum is shown in figure 2 ).
[0039]2. Preparation of miconazole nitrate-modified Na-montmorillonite: Mix 10 g of Na-montmorillonite obtained above with 6 g of miconazole nitrate, add 200 ml of absolute ethanol, and stir the mixture at room temperature for 5 hours. Suction filtration, then wash the filtrate several times with absolute ethanol, detect the absence of miconazole nitrate in the filtrate by ultraviolet spectrophotometer, dry th...
Embodiment 2
[0041] 1. Prepare Na-montmorillonite: with embodiment 1.
[0042] 2. Preparation of miconazole nitrate-modified Na-montmorillonite: Mix 10 g of Na-montmorillonite obtained above with 8 g of miconazole nitrate, add 200 ml of absolute ethanol, and stir the mixture at room temperature for 2 hours. Suction filtration, then wash the filtrate several times with absolute ethanol, detect the absence of miconazole nitrate in the filtrate by ultraviolet spectrophotometer, dry the precipitate in vacuum at 60°C to constant weight, and ball mill until the particle size is 20-30 μm to obtain nitric acid Miconazole modified montmorillonite. All the filtrates were collected and filtered with a 0.45um microporous membrane, and an appropriate amount of the filtrate was used to measure the ultraviolet light absorbance value with a Biomat-5 ultraviolet spectrophotometer, and the remaining drug 6g was calculated. That is, 2 g of miconazole nitrate was intercalated in 10 g of Na-montmorillonite, a...
Embodiment 3
[0044] 1. Prepare Na-montmorillonite: with embodiment 1.
[0045] 2. Preparation of miconazole nitrate-modified Na-montmorillonite: Mix 10 g of Na-montmorillonite obtained above with 10 g of miconazole nitrate, add 200 ml of absolute ethanol, and stir the mixture at room temperature for 5 hours. Suction filtration, then wash the filtrate several times with absolute ethanol, detect the absence of miconazole nitrate in the filtrate by ultraviolet spectrophotometer, dry the precipitate in vacuum at 60°C to constant weight, and ball mill until the particle size is 20-30 μm to obtain nitric acid Miconazole modified montmorillonite. All the filtrates were collected and filtered with a 0.45um microporous membrane, and an appropriate amount of the filtrate was used to measure the ultraviolet light absorbance value with a Biomat-5 ultraviolet spectrophotometer, and the remaining drug 6g was calculated. That is, 4 g of miconazole nitrate was intercalated in 10 g of Na-montmorillonite, ...
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