Method for producing a generation of hybridization seeds of Chinese cabbage by using multiple alleles gene male sterility line

Abstract

A method for producing cabbage F1 hybrid seeds with multiple allele male sterile line relates to the breeding and the manufacturing technology of cabbage multiple allele male sterile line (Brassica campstris L.ssp.pekinensis); the method belongs to the field of utilizing the plant hybrid seeds advantages. The present invention provides a method for producing cabbage F1 hybrid seeds with multiple allele male sterile line. The present invention comprises the following steps that the breeding of the maternal plant cabbage multiple allele male sterile line: the cabbage sterile line is taken as the sterile source and the cabbage fertile line is taken as the breeding material; through the approaches of hybrid, backcrossing and inbreeding, the multiple allele male sterile line is obtained; the breeding of paternal inbred line: the paternal plants are high-generation inbred lines after inbreeding and breeding of a plurality of years of the hybrids of cabbage; F1 hybrid seeds breeding: the cabbage multiple allele male sterile line is hybridized with the paternal inbred lines to get the F1 hybrid seeds.

Description

technical field [0001] The invention relates to the breeding and seed production technology of Chinese cabbage (Brassica campestris L. ssp. pekinensis) polyallelic nuclear gene male sterile line, belonging to the field of crop heterosis utilization. Background technique [0002] Chinese cabbage is rich in varieties and widely distributed. It is an important vegetable crop and occupies an important position in my country's vegetable basket. At present, the production of first-generation hybrid seeds of Chinese cabbage at home and abroad mainly uses self-incompatibility lines for hybrid seed production. This method has the disadvantages of high parental reproduction costs, multi-generation self-bred viability decline, and difficulty in achieving 100% purity of hybrids. question. Secondly, there is also the use of nuclear gene interaction male sterile lines to produce the first generation of hybrids, but because of the poor uniformity of the prepared hybrids, the popularizatio...

AI Technical Summary

Problems solved by technology

At present, the production of first-generation hybrid seeds of Chinese cabbage at home and abroad mainly uses self-incompatibility lines for hybrid seed production. This method has the disadvantages of high parental reproduction costs, multi-generation self-bred viability decline, and difficulty in achieving 100% purity of hybrids. question

Secondly, there is also the use of nuclear gene interaction male sterile lines to produce the first generation of hybrids, but because of the poor uniformity of the prepared hybrids, the popularization and application are greatly affected

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