Anti-senescence product for gonad granulocyte, its preparing method and uses

A granular cell, anti-aging technology, applied in biochemical equipment and methods, anti-toxic agents, microorganisms, etc., can solve the problems of not being able to simulate the physiological state well, the gap between physiological states is large, and the effective ingredients are single, so as to alleviate the symptoms of menopause, Convenient source, high bioavailability effect

Inactive Publication Date: 2008-04-02
ANHUI PROVINCIAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, these additives all use artificially synthesized estrogen, pro

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  • Anti-senescence product for gonad granulocyte, its preparing method and uses

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[0029] 1. Obtain ovarian granulosa cells: 4-6 weeks old female Kunming mice were intraperitoneally injected with 7.5 U of pregnant horse serum (PMSG). After 48 hours of routine rearing, they were killed by cervical dislocation. The bilateral ovaries were taken out under aseptic conditions and placed In a petri dish containing a 4°C precooled balanced salt solution (BSS, containing 400IU / ml hyaluronidase, 100U / ml penicillin and streptomycin), puncture the follicle with a 28G injection needle under a stereo microscope. The granular cells are released into the BSS; the pipette is repeatedly pipetted several times to disperse them into individual cells and filtered through a 200-mesh filter, and centrifuged to wash the cells twice.

[0030] 2. Obtaining granule cell culture supernatant and cell extract: resuspend granule cells in DMEM / F12 medium containing 10% fetal bovine serum by volume and adjust the cell density to 2×10 6 Inoculate the culture flask at 37°C and 5% CO2 volume ratio...

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Abstract

The invention provides a cellsovary granulosa cell anti-aging product, and the preparation method and the application of the product. The invention applies cellsovary granulosa cells in preparation of anti-aging products. The cellsovary granulosa cell anti-aging product of the invention is characterized in that in vitro cultivated and amplified cultivation supernatant of female mammalian animal celsovary granulose cells and/or the cell extract is contained; and one or more than one pharmaceutically acceptable medical excipient and antioxidant are contained. Via the cellsovary granulosa cells, the invention is capable of proliferating and secreting large amounts of a plurality of bioactive substances. The invention adopts granulose cells with extremely high bioavailability and similar to female physiological characteristics to cultivate supernatant and the cell extract of the supernatant. The invention can be taken orally, absorbed by skin or be prepared into vaginal suppository to achieve the purpose of anti-aging.

Description

technical field [0001] The invention relates to an anti-aging product of ovarian granulosa cells, its preparation method and its application. Background technique [0002] The main function of the ovary is to ovulate and secrete female hormones. It controls several important physiological events in a woman's life: youth development, childbearing, maintenance of good figure and beautiful appearance, among which ovarian granulosa cells play an extremely important role . As the age increases, the ovarian function of women begins to decline around the age of 45-50. Some women experience premature ovarian failure due to environmental, psychological or pathological factors, followed by a series of menopausal symptoms, such as menstrual disorders or menopause, emotional changes, muscle Relaxation, decreased elasticity, dullness, accelerated aging, changes in the body's immune function, increased incidence of cardiovascular diseases, osteoporosis, reproductive system tumors, and ne...

Claims

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Application Information

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IPC IPC(8): A61K35/54A61K9/02A61K9/06C12N5/08A61P15/14A61P39/06
Inventor 凌斌冯定庆周颖彭程沈国栋朱园园
Owner ANHUI PROVINCIAL HOSPITAL
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