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Highly effective hydrogen yield photosynthetic bacterium and application thereof

A technology for photosynthetic bacteria and bacteria, applied in the fields of biotechnology and microorganisms, can solve the problems of low conversion efficiency and low hydrogen production efficiency of butyric acid

Inactive Publication Date: 2008-04-30
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there are many hydrogen-producing photosynthetic bacteria isolated from reports at home and abroad, most of the isolated photosynthetic bacteria can use a very narrow range of substrates for hydrogen production, and most of them can only use a small number of organic acids (such as malic acid, acetic acid, Lactic acid, etc.) produce hydrogen efficiently, and the hydrogen production efficiency of butyric acid is generally very low (the conversion efficiency of substrate hydrogen production is lower than 50%)

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  • Highly effective hydrogen yield photosynthetic bacterium and application thereof
  • Highly effective hydrogen yield photosynthetic bacterium and application thereof
  • Highly effective hydrogen yield photosynthetic bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1 Acquisition and characteristic analysis of high-efficiency hydrogen-producing bacterial strains

[0067] In this embodiment, enrichment, separation, purification and optimization of high-efficiency hydrogen-producing strains were carried out according to the following steps, and their characteristics were analyzed.

[0068] (1) Enrichment, separation and purification

[0069] Photosynthetic bacteria were isolated from samples of different natures such as seawater, freshwater, pond water, sewage and mud discharged from factories in Hainan, Hubei, Shaanxi, Shanghai and other parts of the country. The separation medium is the improved RCVB medium, which contains (g / L): 50ml of basic salt solution; 20ml of DL-malic acid (20%, pH6.8); 7.5ml of phosphate buffer; 1.0ml of vitamin solution, sulfuric acid Ammonium or glutamic acid 1g. Among them, the basic salt solution contains: MgSO 4 ·7H 2 O 4g, CaCl 2 ·H 2 O 1.5g, FeSO 4 .7H 2 O 0.236g, Na 2 EDTA 0.4g, tra...

Embodiment 2

[0140] Embodiment 2 Engineering Application Example

[0141] 1. Photosynthetic bacteria ZX-5 used succinic acid wastewater to produce hydrogen by photoanaerobic fermentation in a 6-liter fermenter

[0142] Bacteria ZX-5 was pre-cultured with the improved RCVB medium at 30°C, with a shaker speed of 200rpm / min, and in the dark for 24 hours, and then it could be inoculated into succinic acid wastewater at a 5-7% inoculum size for fermentation Hydrogen production.

[0143] Properly dilute the succinic acid wastewater (containing 1.75% succinic acid) retrieved from Shanghai Shenren Succinic Acid Factory to about 50mM (the concentration of succinic acid is about 1.35%), and then add the nutrients required for the growth of photosynthetic bacteria ZX-5 Elements (that is, all components in the improved RCVB medium that omits DL-malic acid) adjust the pH to about 7.0, and use photoanaerobic conditions under the conditions of light 4000-5000 lux, temperature 28-31°C, and rotation speed...

Embodiment 3

[0154] Embodiment 3 Utilizes sugar or alcohols to produce hydrogen

[0155] Use 0.4% glucose and mannitol as substrates, and the rest of the nutrients are modified RCVB medium without adding malic acid. Under the conditions of light 4000lux and temperature 30°C, carry out photoanaerobic fermentation to produce hydrogen in a 38ml test tube. In the experiment, the maximum hydrogen production rates of the two substrates (glucose and mannitol) were 105mlH 2 / L·h, 110mlH 2 / L·h, the gases produced are hydrogen and carbon dioxide, and the conversion efficiencies of the two substrates are 50% and 65%, respectively. The remaining substrate is used for cell growth.

[0156] After the hydrogen production by photofermentation is over, almost no glucose and mannitol are detected in the fermentation broth. Therefore, after comprehensive utilization of the bacteria, the fermentation broth can be directly discharged up to the standard.

[0157] Culture preservation

[0158] The Rhodobact...

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Abstract

The invention discloses hydrogen production photosynthetic bacteria, which has wide substrate spectra production range and can efficiently convert different organic substances into hydrogen. The hydrogen production photosynthetic bacteria which can efficiently convert different organic substances into hydrogen can be widely applied to the process of different organic substances and the light fermentation hydrogen production in organic waste water with carbohydrate as the substrate. The organic waste water can be directly discharged after being processed by bacterial treatment of the invention according to standards, thereby the invention is in favor of the comprehensive utilization of the waste water, reduces the environmental contamination, and simultaneously greatly reduces the cost of biologic hydrogen production.

Description

technical field [0001] The invention belongs to the field of biotechnology and microorganisms, in particular, the invention relates to a high-efficiency hydrogen-producing photosynthetic bacterium and its application. Background technique [0002] In actual industrial production, the wastewater produced by many factories is rich in organic acids, such as malic acid factories, succinic acid factories, vinegar factories, butyric acid factories, and the fermented liquid produced by dark fermentation of waste. low molecular weight organic acids. More and more studies have proved that photosynthetic bacteria can use various organic acids to produce hydrogen by photofermentation. This can not only improve the substrate conversion efficiency of dark fermentation of waste to produce hydrogen, and obtain more hydrogen, but also solve the environmental pollution problem caused by high-concentration organic wastewater in factories. The hydrogen production capacity (hydrogen productio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P3/00C02F3/34
Inventor 周志华陶用珍陈杨吴永强
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI