Method for preparing immobilization microorganism particles using pottery ball as carrier

A technology for immobilizing microorganisms and microorganisms, applied in the direction of immobilization on or in inorganic carriers, biological sludge treatment, etc., can solve the problems of unreported sediment restoration, and achieve practical safety, reliability, good effect and low price. Effect

Inactive Publication Date: 2008-04-30
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

There have been some successful examples of remediation of polluted water...

Method used

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  • Method for preparing immobilization microorganism particles using pottery ball as carrier
  • Method for preparing immobilization microorganism particles using pottery ball as carrier

Examples

Experimental program
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Effect test

Embodiment 1

[0027] The method for immobilizing microorganisms with pottery beads comprises the following steps: firstly, seed liquid is prepared, and inorganic carrier pottery beads are treated simultaneously; Specific steps are as follows:

[0028] (1) Preparation of seed solution

[0029] Under facultative anaerobic conditions, add 160ml of enriched medium to a 250ml anaerobic bottle, sterilize, add 18ml of seed solution after cooling, and put it in an incubator at 28°C for constant temperature and static culture for one week, so that the microorganisms are in the right condition. Number of growing seasons, spare.

[0030] The anaerobic bottle is a medical infusion bottle; the culture medium is calculated by weight percentage: glucose 0.1%, peptone 0.05%, yeast extract 0.05%, NaCl 0.5%, K 2 HPO 4 0.5%, KH 2 PO 4 0.3%, MgSO 4 ·7H 2 O 0.05%, pH7.2. The sterilization conditions are: 0.1Mpa damp heat sterilization for 30 minutes. Described seed liquid is the facultative anaerobic...

Embodiment 2

[0040] The difference from Example 1 is:

[0041] (1) Preparation of seed solution

[0042] Under facultative anaerobic conditions, add 180ml of enriched medium into a 250ml anaerobic bottle, sterilize, add 16ml of seed solution after cooling, and put it in an incubator at 28°C for a week of constant temperature and static culture, so that the microorganisms are in the right condition. Number of growing seasons, spare.

[0043] The medium is calculated by weight percentage: glucose 0.2%, peptone 0.06%, yeast extract 0.06%, NaCl 0.6%, K 2 HPO 4 0.5%, KH 2 PO 4 0.3%, MgSO 4 ·7H 2 O 0.05%, pH7.2. Sterilization, the sterilization conditions are: 0.1Mpa damp heat sterilization for 30 minutes. The seed liquid is a facultative anaerobic mixed microorganism containing 2ml of nitrobenzene solution with a concentration of 3mg / ml, and the concentration of nitrobenzene is 30.30mg / L.

[0044] (2) Pretreatment of the carrier

[0045] Pottery beads (purchased from Jinzhou Daye Ce...

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Abstract

The invention relates to the restoration technology of bottom mud in polluted water bodies, in particular to a method for preparing immobilized microbial particles with pottery beads as carriers. Specifically, the prepared seed solution is inoculated into the treated ceramic bead carrier, and under the condition of facultative anaerobic and constant temperature culture for 4-6 days, the adsorption, cultivation and proliferation are carried out 2-3 times, and the microorganisms immobilized are obtained. of ceramic beads. The invention specifically adopts inorganic carrier ceramic beads to adsorb and fix microorganisms, has fast adsorption speed to mixed bacteria, good multiplication effect, simple operation, no need to change natural environment and build large-scale structures, and does not generate secondary pollution. Putting the immobilized cell particles prepared by it into the nitrobenzene polluted water body-sediment environment can effectively remove nitrobenzene and aniline in the sediment. When the initial concentration of nitrobenzene and aniline in the sediment is 4.41mg /kg, 37.84mg/kg, the degradation rate of nitrobenzene can reach 100%, and the degradation rate of aniline is 66%. The pottery beads of the present invention are rich in sources and cheap in price.

Description

technical field [0001] The invention relates to the restoration technology of bottom mud in polluted water bodies, in particular to a method for preparing immobilized microbial particles with pottery beads as carriers. Background technique [0002] Conventional environmental remediation technologies mainly refer to chemical remediation, physical remediation, ecological remediation and bioremediation. The latter two are often classified as unified technologies, and in-situ methods are generally adopted in engineering. [0003] The special properties of sediment in polluted water increase the difficulty of physical and chemical remediation. Bioremediation is the use of plants, animals and microorganisms to enrich, absorb and degrade pollutants. The restoration process can be carried out naturally and in situ. The bioremediation described in this technology mainly refers to microbial remediation. [0004] On November 13, 2005, an explosion occurred in the aniline workshop of ...

Claims

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Application Information

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IPC IPC(8): C12N11/14C02F11/02
Inventor 李培军鞠京丽张海荣张春桂许华夏
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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