Immobilized fibrous bed bioreactor for fermentation productions of ethylformic acid, butyric acid and succinic acid

A reactor and fiber bed technology, which is used in the fermentation production of succinic acid, propionic acid, butyric acid, and immobilized fiber bed reactors, can solve the problem that cells are difficult to achieve self-renewal, not suitable for large-scale production of propionic acid, and unsuitable for large-scale production of propionic acid. Live cell fermentation and other problems, to achieve the effect of strong adaptability and organic acid production capacity, low cost, and simplified equipment

Active Publication Date: 2008-05-21
NANJING TECH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limitation of mass transfer conditions, it is difficult for the cells embedded in the gel embedding method to achieve self-renewal, and it is difficult to ensure the viability of the bacteria for a long time, so it is not suitable for live cell fermentation
In addition, gel embedding is expensive and not suitable for large-scale production of propionic acid

Method used

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  • Immobilized fibrous bed bioreactor for fermentation productions of ethylformic acid, butyric acid and succinic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 The method for producing propionic acid

[0034] Propionibacterium freudeenreichii NX-4 (Propionibacterium freudeenreichii NX-4, patent application number: 200710020579.8) which has been patented by our laboratory is used to produce propionic acid in an immobilized fiber bed reactor. Seed medium: glucose 20g / l, peptone 5g / l, yeast extract 5g / l, NaCl 5g / l, pH 6.9. Fermentation medium: corn steep liquor 40g / l, (NH 4 ) 2 SO 4 2g / l, K 2 HPO 4 5g / l, NaCl 1g / l, pH 6.9.

[0035] The plant fiber (gauze) 9 is fixed in the reactor 10, and the P. freundii NX-4 seed solution is inserted into the reactor 10 pre-loaded with 3L seed culture medium at an inoculum size of 5% (v / v). , temperature 35°C, feed 0.1L / min N from inert gas inlet 4 2 , using the stirring device 11 to stir (rotating speed 200r / min), while using the constant flow pump 5-1 to fill the 8mol / l sodium hydroxide solution in the lye bottle 2 into the reactor 10 to control the pH 6.0, and cultivate ...

Embodiment 2

[0036] Embodiment 2 The method for producing propionic acid

[0037] Production of propionic acid in an immobilized fiber bed reactor using Propionibacterium fresnerei NX-4. Seed medium and fermentation medium are the same as in Example 1. The animal fiber (wool fabric) 9 is fixed in the reactor 10, and the P. freundii NX-4 seed solution is inserted into the reactor 10 pre-loaded with 3L seed medium by the inoculation amount of 15% (v / v). , feed 0.1L / min N from the inert gas inlet 4 2 , using the stirring device 11 to stir (rotating speed 300r / min), while using the constant flow pump 5-1 to add 3mol / l sodium hydroxide solution to control the pH 6.0, and culture for 48h to realize the immobilization of the cells on the fiber 9. Replace the seed solution in the reactor 10 with the fermentation medium (initial glucose concentration 120g / l), the initial pH is 6.9, use the constant flow pump 5-1 to add 3mol / l sodium hydroxide solution to control the pH 6.0, and the temperature is...

Embodiment 3

[0038] Embodiment 3 The method for producing propionic acid

[0039] Production of propionic acid in an immobilized fiber bed reactor using Propionibacterium fresnerei NX-4. Seed medium and fermentation medium are the same as in Example 1. Synthetic fiber (polyester fabric) 9 is fixed in the reactor 10, by the inoculum size of 10% (v / v) the reactor 10 that Propionibacterium frescherii NX-4 seed solution is inserted in advance and is equipped with 3L seed culture medium , feed 0.1L / min N from the inert gas inlet 4 2 , use the stirring device 11 to stir (speed 600r / min), and use the constant flow pump 5-1 to add 6mol / l sodium bicarbonate solution to the reactor 10 to control the pH 6.0 and cultivate for 40h to realize the immobilization of the cells on the fiber 9 . The seed liquid in the reactor 10 is replaced with fermentation medium (initial glucose concentration), and initial pH is 6.9, utilizes constant flow pump 5-1 to add 6mol / l sodium bicarbonate solution to control p...

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Abstract

The invention relates to an immobilized fibrous bed reactor for fermentation production of propionic acid, butyric acid and amber acid. The reactor mainly comprises a reactor (10), fiber material (9), a stirring device (11), a pH automatic control device and a feeding device. The invention is characterized in that the fiber material (9) is fixed in the reactor (10), which is used as carrier for cell immobilization; the pH automatic control device and the feeding device are connected with the reactor (10); the stirring device (11) is suspended in an inner part of the reactor (10). The reactor is used to produce propionic acid, butyric acid and amber acid with high production efficiency and stable batch production. And the device has the advantages of cheap immobilization material, simple arrangement and convenient operation, which are suitable for the industrial production of propionic acid, butyric acid or amber acid.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering and relates to an immobilized fiber bed reactor, which is used for the fermentation production of propionic acid, butyric acid and succinic acid. Background technique [0002] Organic acids refer to a class of compounds with a carboxyl group (-COOH) in their molecular structure, and have broad application prospects in the fields of food, feed, and plastics. Traditional organic acid production methods mainly use petroleum as raw material to produce by chemical synthesis. At present, the production of organic acids by biological methods has been favored more and more due to the advantages of natural products and abundant raw materials. However, there are still many problems in organic acid fermentation production. Organic acid microbial fermentation, such as propionic acid, butyric acid, and succinic acid fermentation production, generally has problems such as slow bacterial produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/02C12M1/36C12P7/52C12P7/46C12P7/40
CPCC12M25/18C12M41/26C12M27/02
Inventor 徐虹冯小海李凯李莎欧阳平凯
Owner NANJING TECH UNIV
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