Urine process method

A treatment method, urine technology, applied in the direction of material inspection products, preparation of test samples, biological testing, etc., can solve the problems of inapplicable quantitative detection, etc., and achieve the effect of simple operation

Inactive Publication Date: 2008-05-21
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are only applicable to the examination of urine sediment, mainly for the detection of formed elements in urine, such as white blood cells, red blood cells, casts, epithelial cells, crysta

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0027] Collect 15ml of fresh morning urine from patients with calculus and normal persons, and immediately add 0.15ml of 2% (mass fraction) sodium azide solution to the collected fresh morning urine from patients with calculus and normal persons for preservation, and then add 15ml of anhydrous Ethanol, let it stand for 30 minutes to denature the protein in the urine; then filter through a 1.2 μm microporous membrane to remove cell debris and denatured protein precipitation, take the filtrate, drop the filtrate on a clean glass piece, and place it in a dust-free incubator. The temperature is controlled at about 50°C, the urine is allowed to volatilize, and the urine microcrystals are retained for XRD characterization. The results are shown in Figure 1. Figure 1 is the XRD spectrum of the urine sample treated in this example, where a and b are normal human urine The XRD pattern of urine microcrystals in urine, c, d are the XRD patterns of urine microcrystals in the urine of patie...

Embodiment 2

[0029] Collect 15ml of fresh morning urine from patients with calculi and normal persons respectively, and immediately add 0.30ml of 1% (mass fraction) sodium azide solution to the fresh morning urine collected from patients with calculus and normal persons for preservation, and then add 15ml of anhydrous Ethanol, let it stand for 40 minutes to denature the protein in the urine; centrifuge (4000 rpm) for 10 minutes to remove cell debris and denatured protein precipitates, take the supernatant and drop it on a copper grid, and do TEM to detect the shape and size of the crystal after drying , the results are shown in Fig. 2, Fig. 2 is the TEM figure of the urine sample processed in this embodiment, wherein a is the TEM figure of the urine microcrystals in the urine of normal people, and wherein b is the TEM figure of the urine microcrystals in the urine of patients with urolithiasis From Figure 2, it can be seen that there are obvious differences in the TEM pictures of urine crys...

Embodiment 3

[0031] Collect 15ml of fresh morning urine from patients with calculus and normal persons, and immediately add 0.30ml of 2% (mass fraction) sodium azide solution to the fresh morning urine of patients with calculus and normal persons for preservation, and then add 30ml of anhydrous Ethanol, let it stand for 30 minutes to denature the protein in the urine; then filter through a 0.5 μm microporous membrane to remove cell debris and denatured protein precipitates, and dilute the filtrate by 20% with 0.15 mol / L NaCl solution (normal saline) , drop it on a clean glass sheet, place it in a dust-free incubator, and control the temperature at about 50°C. After the urine is volatilized, the sample is sprayed with gold, and the urine microcrystals are characterized by SEM. The results are shown in Figure 3. Fig. 3 is the SEM figure of the urine sample that present embodiment processes, and wherein a is the SEM figure of urine crystallite in the urine of normal person, and wherein b is th...

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Abstract

The invention relates to material separation, and specifically provides a method for treating urine. The urine treatment method comprises the following steps: collecting the urine, adding a sodium azide solution with a mass fraction of 1% to 3%, and the amount of the sodium azide solution added is 0.2% to 4% of the volume of the urine, Then add dehydrated ethanol, the volume ratio of dehydrated ethanol added and urine is urine:dehydrated alcohol=1~3:1, after standing for 20~60 minutes, adopt 0.45~1.2μm membrane suction filtration or Centrifugation to remove cellular debris and protein in the urine is sufficient. The urine treated by the present invention can be used to correctly detect the composition, shape and size distribution of nanometer and submicron crystals in urine, and according to the composition, shape and size distribution of urine crystals. Simultaneously, the method of the present invention is simple, accurate and fast in operation, and can provide scientific basis for finding the cause of calculus formation, prevention and treatment in clinic.

Description

technical field [0001] The invention belongs to the technical field of material separation, and in particular relates to a method for treating urine. Background technique [0002] Urinary calculus (also known as urolithiasis) is a common and frequently-occurring disease worldwide, and its incidence is on the rise. Urolithiasis occupies the first place in urology inpatients, more than 40%. Especially in my country's Guangdong Province and Hong Kong and Macao regions, submicron, like nasopharyngeal carcinoma, has become a serious endemic disease in Guangdong Province and Hong Kong and Macao regions, bringing great suffering to people. In Florida, USA, the number of stone patients was 15.7 / 100,000 in 1987 and 20.8 / 100,000 in 1996, an increase of 32.5%. In Dongguan, Guangdong, it was 101 / 100,000 people in the late 1980s, and rose to 140 / 100,000 people in the 1990s, an increase of 38.6%, but the actual prevalence rate is much higher than this. [0003] Urolithiasis has a high ...

Claims

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Application Information

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IPC IPC(8): G01N1/34G01N33/48
Inventor 欧阳健明万牡华
Owner JINAN UNIVERSITY
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