Urine process method

A treatment method, urine technology, applied in the direction of material inspection products, preparation of test samples, biological testing, etc., can solve the problems of inapplicable quantitative detection, etc., and achieve the effect of low noise and simple operation

Inactive Publication Date: 2010-05-26
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are only applicable to the examination of urine sediment, mainly for the detection of formed elements in urine, such as white blood cells, red blood cells, casts, epithelial cells, crystals and salts, etc., which are generally used for routine medical examinations and are not suitable for urine Quantitative detection of the content and concentration of certain substances in components or urine

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Experimental program
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Effect test

Embodiment 1

[0027] Collect 15ml of fresh morning urine from patients with calculus and normal persons, and immediately add 0.15ml of 2% (mass fraction) sodium azide solution to the collected fresh morning urine from patients with calculus and normal persons for preservation, and then add 15ml of anhydrous Ethanol, let it stand for 30 minutes to denature the protein in the urine; then filter through a 1.2 μm microporous membrane to remove cell debris and denatured protein precipitation, take the filtrate, drop the filtrate on a clean glass piece, and place it in a dust-free incubator. The temperature is controlled at about 50°C, the urine is allowed to volatilize, and the urine microcrystals are retained for XRD characterization, the results are shown in figure 1 , figure 1 It is the XRD spectrum of the urine sample processed in this embodiment, wherein a, b are the XRD spectrum of urine microcrystals in normal human urine, c, d are the XRD spectrum of urine microcrystals in the urine of p...

Embodiment 2

[0029] Collect 15ml of fresh morning urine from patients with calculi and normal persons respectively, and immediately add 0.30ml of 1% (mass fraction) sodium azide solution to the fresh morning urine collected from patients with calculus and normal persons for preservation, and then add 15ml of anhydrous Ethanol, let it stand for 40 minutes to denature the protein in the urine; centrifuge (4000 rpm) for 10 minutes to remove cell debris and denatured protein precipitates, take the supernatant and drop it on a copper grid, and do TEM to detect the shape and size of the crystal after drying , see the result figure 2 , figure 2 It is the TEM figure of the urine sample processed in this embodiment, wherein a is the TEM figure of urine microcrystals in normal human urine, and wherein b is the TEM figure of urine microcrystals in the urine of patients with urolithiasis, by figure 2 It can be seen that there are obvious differences in the TEM images of normal human urine crystals...

Embodiment 3

[0031] Collect 15ml of fresh morning urine from patients with calculus and normal persons, and immediately add 0.30ml of 2% (mass fraction) sodium azide solution to the fresh morning urine of patients with calculus and normal persons for preservation, and then add 30ml of anhydrous Ethanol, let it stand for 30 minutes to denature the protein in the urine; then filter through a 0.5 μm microporous membrane to remove cell debris and denatured protein precipitates, and dilute the filtrate by 20% with 0.15 mol / L NaCl solution (normal saline) , drop it on a clean glass sheet, put it in a dust-free incubator, and control the temperature at about 50°C. After the urine is volatilized, the sample is sprayed with gold, and the urine microcrystals are characterized by SEM. The results are shown in image 3 , image 3 It is the SEM figure of the urine sample processed in the present embodiment, wherein a is the SEM figure of urine microcrystals in normal human urine, and wherein b is the S...

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Abstract

The present invention relates to material separation, in particular to a urine treatment method. The urine treatment method comprises the steps as follows: the urine is collected; sodium azide solution with a quality fraction of 1 percent to 3 percent is added in the urine, the quantity of the added sodium azide is 0.2 percent to 4 percent of the bulk of the urine; then absolute alcohol is added,the volume ratio of the absolute alcohol to the urine is from 1 : 1 to 3 : 1; after 20 to 60 minutes of standing, the cell debris and the protein in the urine are eliminated by 0.45 Mu m to 1.2 Mu m filtration membrane extraction filtration or centrifugation. The composition, the morphology and the size distribution of the nanometer and the submicron crystal in the urine treated by the present invention can be accurately tested according to the composition, the morphology and the size distribution of the urine crystal. At the same time, the method of the present invention is of simple, accurate and quick operation. The present invention can provides a scientific basis for seeking stone cause, prevention and treatment in clinic.

Description

technical field [0001] The invention belongs to the technical field of material separation, and in particular relates to a method for treating urine. Background technique [0002] Urinary calculus (also known as urolithiasis) is a common and frequently-occurring disease worldwide, and its incidence is on the rise. Urolithiasis occupies the first place in urology inpatients, more than 40%. Especially in my country's Guangdong Province and Hong Kong and Macao regions, submicron, like nasopharyngeal carcinoma, has become a serious endemic disease in Guangdong Province and Hong Kong and Macao regions, bringing great suffering to people. In Florida, USA, the number of stone patients was 15.7 / 100,000 in 1987 and 20.8 / 100,000 in 1996, an increase of 32.5%. In Dongguan, Guangdong, it was 101 / 100,000 people in the late 1980s, and rose to 140 / 100,000 people in the 1990s, an increase of 38.6%, but the actual prevalence rate is much higher than this. [0003] Urolithiasis has a high ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/34G01N33/48
Inventor 欧阳健明万牡华
Owner JINAN UNIVERSITY
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