Application of cell chip in gene toxicology and animal toxicology

A cell chip and animal poisoning technology, applied in tissue cell/virus culture devices, biological testing, optical devices, etc., can solve problems such as lack of liver donors

Inactive Publication Date: 2008-05-28
泰州伯克利生物技术有限公司 +1
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  • Abstract
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  • Claims
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Problems solved by technology

However, there are many restrictions on liver transplantation, not all patients have the opportunity to wait for liver replacement; moreover, there is a shortage of liver donors, and liver tissue cultured in vitro can be used as a bridge to help patients with end-stage liver disease, allowing patients to prolong life life waiting for a suitable liver donor, therefore, the development of a successful artificial liver support system will be clinically important

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  • Application of cell chip in gene toxicology and animal toxicology

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Embodiment Construction

[0008] The present invention relates to the application of a cell chip in gene toxicology and animal toxicology. The cell chip is also called artificial liver. The three-dimensional cell culture in the chip described in the present invention forms the structure and function in the body. The cell culture chip in the chip creates condition control for extracellular culture, so as to realize long-term survival of cells.

[0009] 1. Device design and production process:

[0010] 1. First fabricate a microfluidic device equipped with temperature control and fluidic integrated components. It roughly consists of two parts. The first part is made of polymer material PDMS. PDMS is an optically transparent material and can be permanently adhered to the smooth surface of silicon wafers. Microfluidic channels and surface electrodes are made on the surface of the chip by photolithography. The central part of the PDMS chip is removed to serve as small cell culture dishes. These microflui...

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Abstract

The invention discloses application of artificial livers in gene toxicology and animal toxicology, which enables liver tissues to form conditions of structure, PH values, nutrition, temperature, concentration of CO2 and the like which are analogous to those inside human body on cell chips, thereby the liver tissues can live for long on cell chips, expressions of functional genes of liver cells such as Albumin and HNF4 reach a level similar to that inside human body, large-scaled cultivation of liver cell chips is achieved, reaction of artificial livers in toxicology and pharmacology is quantified, and comparison of reaction among artificial livers, animal experiments and excised livers, and external experiments of liver cells to medicine is made, and existing experiments on live animals can be replaced.

Description

technical field [0001] The invention relates to the application of a cell chip in gene toxicity and animal toxicity. Background technique [0002] At present, only a small number of biological instrument companies and university research departments in the United States are conducting research on cell chip technology in the world. These companies have already had certain prototype products in the field of cell tissue culture chip instruments, but most of these products are not based on human cells. However, non-human cells such as pigs are used as research materials, and the price and efficiency of the current products cannot meet the actual needs, so there is an urgent need to develop a new generation of products. Liver tissue engineering research has been carried out in laboratories in the United States and Japan for many years. At present, there are a few domestic laboratories engaged in research in this area, but none are engaged in research on human liver cells. Combin...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12Q1/68G01N33/50G01N21/64G01B11/00
Inventor 徐景宏徐韧黄金麟
Owner 泰州伯克利生物技术有限公司
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