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Color matching detecting and analyzing method of cobalt ion

An analysis method and technology of cobalt ions, applied in the field of colorimetric detection and analysis of cobalt ions, achieves the effects of fast color change, simple preparation process, and convenient solution preparation

Inactive Publication Date: 2008-06-11
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

Using charge-transfer complexes as detection probes, cobalt ions (Co 2+ ) There is no relevant literature report

Method used

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  • Color matching detecting and analyzing method of cobalt ion

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Embodiment 1

[0033] Preparation of detection reagent: Accurately weigh a certain amount of 7,7,8,8-tetracyanoquinodimethane (TCNQ) (concentration 5.0×10 -4 M) and a certain amount of 8-hydroxyquinoline (concentration 5.0×10 -3 M), adding acetonitrile to dissolve, constant volume, the solution turns green for subsequent use. This green solution is the detection reagent for the detection of cobalt ions.

[0034] Preparation of the metal ion test solution: Weigh Li + , Na + , K + , Mg 2+ , Ca 2+ , Ba 2+ , Zn 2+ , Cu 2+ , Hg 2+ , Cd 2+ , Pb 2+ , Ni 2+ and Co 2+ The nitrates of 13 kinds of metal ions are placed in the volumetric flask with a concentration of 2.5×10 -3 M was dissolved in HEPES aqueous solution, and the volume was constant. The concentration of various metal ions to be tested is 1.0×10 -4 M.

[0035] Colorimetric detection of metal ions: 2ml of various metal ion buffer solutions (1.0×10 -4 M) with 2ml of TCNQ / 8-hydroxyquinoline (TCNQ=5.0×10 -4 M, 8-hydroxyquino...

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Abstract

The invention relates to a color comparison testing and analyzing method of cobalt ion. The method uses 8-hydroxyquinoline as electron donor, 7, 7, 8, 8-tetracyanoquinodimethane as electron acceptor. Colored charge transfer complex is formed in polar solvent through the charge transferring effect among molecule and is made as testing reagent. Water-soluble cobalt salt are made into water solution of definite viscosity, a definite volume of water solution and the testing reagent with same volume are mixed. The color of the solution changes obviously, and other metal ion doesn't interfere cobalt ion. With room temperature testing, the color comparison reaction of the testing reagent to cobalt ion can be identified by eyes or tested by using ultraviolet-visible spectrophotometer. The invention has very professional color comparison identifying capability to cobalt ion the preparation of solution is convenient, the operation of the color comparison is very simple, and the color changing speed of color comparison reaction is quick, which can finish the color comparison test to the tested object in very short time.

Description

technical field [0001] The present invention relates to a kind of cobalt ion (Co 2+ ) colorimetric detection analysis method. technical background [0002] The detection and analysis of metal ions has important applications in environmental monitoring, wastewater treatment, biology, clinical medicine and other fields. [0003] Cobalt is the No. 27 element. It is an important element both in industry and in biological systems. It is necessary for all life systems. For the human body, it is vitamin B 12 In addition, cobalt is also involved in the synthesis of proteins, the storage of folic acid, the activation of thiol enzymes and the formation of myelin. However, animal experiments have shown that high concentrations of cobalt are also possible carcinogens, and cobalt poisoning can cause pulmonary dysfunction, dermatitis, nausea and vomiting. Therefore, the development of a convenient, sensitive and rapid detection of cobalt ions (Co 2+ ) means is very necessary. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/84
Inventor 邵士俊胡树振郭勇徐健
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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