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Method for producing ferulic acid esterase by solid fermentation

A technology of ferulic acid esterase and fermentation medium, which is applied in the field of fermentation industry, can solve the problems of high cost, low ferulic acid esterase activity, waste water pollution, etc., and achieve the goals of improving utilization rate, vigorous growth and reducing production cost Effect

Inactive Publication Date: 2008-06-18
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, purified enzymes are used in research, and the cost is high
[0004] At present, the production method of ferulic acid esterase is mainly based on liquid fermentation, and the activity of the obtained ferulic acid esterase is relatively low, and waste water, etc. cause great pollution
[0005] Therefore, the present invention aims to study a method for producing ferulic acid esterase by solid-state fermentation of steam-exploded straw, so as to overcome the shortcoming of low ferulic acid esterase activity, and at the same time effectively utilize lignocellulose resources to realize full utilization of its biomass

Method used

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  • Method for producing ferulic acid esterase by solid fermentation
  • Method for producing ferulic acid esterase by solid fermentation
  • Method for producing ferulic acid esterase by solid fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Production of ferulic acid esterase by steam-exploded wheat straw solid-state fermentation

[0023] 1. Steam-exploded wheat straw

[0024] Add 150g of wheat straw cut to a length of 10-15cm, add 150ml of water, stir evenly, put it into a steam explosion tank, and feed steam. After the cold air in the tank is discharged, close the charging valve, increase the pressure to 1.6MPa, and maintain the pressure for 4 minutes. Open the discharge valve and release the instant decompression to get the steam-exploded wheat straw.

[0025] 2. Preparation and sterilization of fermentation medium

[0026] Add 1g of bran to 4g of steam-exploded wheat straw, add water to a solid-to-liquid ratio of 1:4, stir evenly, and keep the natural pH value in a sterilizing pot at 121°C for 30min.

[0027] 3. Vaccination

[0028] The sterilized culture medium was taken out, and 5 ml of Aspergillus niger liquid seeds obtained by screening were inserted into the ultra-clean bench, and...

Embodiment 2

[0032] Example 2: Ferulic esterase produced by steam-exploded rice straw solid-state fermentation

[0033] 1. Steam-exploded straw

[0034] Add 150g of straw cut to a length of 10-15cm, add 150ml of water, stir evenly, put it into a steam explosion tank, and feed steam. After the cold air in the tank is discharged, close the filling valve, increase the pressure to 1.6MPa, and maintain the pressure for 4 minutes. Open the discharge valve and release the instant decompression to get the steam-exploded straw.

[0035] 2. Preparation and sterilization of fermentation medium

[0036] Add 1g of bran to 4g of steam-exploded rice straw, add water to a solid-to-liquid ratio of 1:4, stir evenly, and maintain a natural pH value in a sterilizing pot at 121°C for 30min.

[0037] 3. Vaccination

[0038] The sterilized culture medium was taken out, and 5 ml of Aspergillus niger liquid seeds obtained by screening were inserted into the ultra-clean bench, stirred evenly, and cultured in a 3...

Embodiment 3

[0042] Example 3: Production of ferulic acid esterase by solid-state fermentation of steam-exploded corn stalks

[0043] 1. Steam-exploded corn stalks

[0044] Add 150g of corn stalks cut to a length of 3-5cm, add 150ml of water, mix evenly, put it into a steam explosion tank, and feed in steam. After the cold air in the tank is exhausted, close the charging valve and increase the pressure to 1.6MPa. After 4 minutes, open the discharge valve, and release the pressure instantly to get the steam-exploded corn stalks.

[0045] 2. Preparation and sterilization of fermentation medium

[0046] Add 1g of bran to 4g of steam-exploded corn stalks, add water to a solid-to-liquid ratio of 1:4, stir evenly, and maintain a natural pH value in a sterilizing pot at 121°C for 30min.

[0047] 3. Vaccination

[0048] The sterilized culture medium was taken out, and 5 ml of Aspergillus niger liquid seeds obtained by screening were inserted into the ultra-clean bench, stirred evenly, and cultu...

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Abstract

The present invention belongs to the technical field of fermentation industry and especially relates to a method of producing ferulic acid esterase. The method is that water is added into 75 percent to 100 percent (weight percentage) of plant straws which are processed by steam explosion and 0 percent to 25 percent (weight percentage) of bran to be made into fermentation culture medium, the solid-liquid ratio is 1 : 1 to 10; pH value is adjusted to be 3.0 to 11.0; the sterilization is processed; liquid seeds are accessed to be fermented for three to four days; a fermented product is extracted by water; supernatant is ferulic acid esterase liquid. The fermentation medium used by the present invention has abundant resource and low price; the fermentation manner is simple; the obtained ferulic acid esterase has high activity.

Description

Technical field: [0001] The invention belongs to the technical field of fermentation industry, in particular to a method for producing ferulic acid esterase by solid-state fermentation. Background technique: [0002] Ferulic acid is an aromatic organic acid widely present in plant cell walls, and is one of the active ingredients of many Chinese herbal medicines such as Ferulicum and Angelica. It has been widely used in medicine, food, cosmetics and other industries. However, the content of ferulic acid in plant cell walls is relatively low, and most of them are combined between lignin and polysaccharides in the form of monomers or aggregates, making the structure of cell walls more complex and dense, and increasing the difficulty of cellulase hydrolysis. Most of its extraction processes use chemical methods, which generate a large amount of organic reagent wastewater, and the cost is relatively high. [0003] Ferulic acid esterase is a kind of carboxylesterase which was is...

Claims

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Application Information

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IPC IPC(8): C12N9/18C12N1/14C12R1/685
Inventor 陈洪章王洪川
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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